Heterogeneous shedding of Brucella abortus in milk and its effect on the control of animal brucellosis
Heterogeneous shedding of Brucella abortus in milk and its effect on the control of animal brucellosis
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DOI：
10.1111/j.1365-2672.2009.04177.x
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发表时间：
2009-06-01
影响因子：
4
通讯作者：
Iannelli, D.
中科院分区：
文献类型：
--
作者：
Capparelli, R.;Parlato, M.;Iannelli, D.
目的：为了确定在牛种布氏杆菌感染的布法罗群中，是否可以通过扑杀超级传播者（每毫升牛奶中分泌>= 10（4）CFU的动物）来减少新感染的动物数量。用培养法测定了500头水牛乳汁中牛种的菌落形成单位（CFU/ml）。每只动物每隔一个月测试三次。B的存在与否。通过PCR确认每个乳汁样品中的流产。大多数受感染的动物以低水平（= 1（0）4 CFU ml（-1））排出病原体。B.流产阳性乳汁样本的结果与培养结果相当。扑杀的超级传播者是足够的逮捕infection.Significance和影响的研究：这里描述的方法可以显着降低控制布鲁氏菌病的成本。培养和定量PCR检测可以识别超级传播者，与用于检测布鲁氏菌病的血清学检测相比，还可以更准确地估计疾病的发病率。
Aims: To ascertain whether in Brucella abortus-infected water buffalo herds, the number of newly infected animals could be reduced by culling superspreaders (the animals secreting >= 10(4) CFU per ml of milk).Methods and Results: The number of B. abortus present in the milk (CFU per nil) from 500 water buffaloes was measured by the Culture. Each animal was tested three times, at one month intervals. The presence or the absence of B. abortus in each milk sample was confirmed by PCR. A majority of infected animals shed the pathogen at a low level (= 1(0)4 CFU ml(-1)). Quantitative PCR of B. abortus positive milk samples gave comparable results to Culture. Culling of the superspreaders was sufficient to arrest the spread of infection.Significance and Impact of the Study: The approach described here can reduce significantly the cost of controlling brucellosis. Culture and quantitative PCR tests identify superspreaders and, compared with the serological tests in use to detect brucellosis, provide also a more accurate estimate of the disease incidence.