An Open-Labeled Study on Fecal Microbiota Transfer in Irritable Bowel Syndrome Patients Reveals Improvement in Abdominal Pain Associated with the Relative Abundance of Akkermansia Muciniphila
An Open-Labeled Study on Fecal Microbiota Transfer in Irritable Bowel Syndrome Patients Reveals Improvement in Abdominal Pain Associated with the Relative Abundance of Akkermansia Muciniphila
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DOI：
10.1159/000494252
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发表时间：
2019-01-01
期刊：
影响因子：
3.2
通讯作者：
Stein-Thoeringer, Christoph K.
中科院分区：
文献类型：
--
作者：
Cruz-Aguliar, Rebeca M.;Wantia, Nina;Stein-Thoeringer, Christoph K.
背景/目的：肠道微生物群在肠易激综合征（IBS）中发生改变，通过饮食或抗生素控制微生物群可以减轻其症状。由于粪便微生物群转移 (FMT) 在 IBS 中的应用仍存在争议，因此我们在一组患有复发性难治性症状的 IBS 患者中研究了 FMT 的临床和副作用，并研究了肠道微生物群特征。方法：采用观察性、前瞻性研究设计，我们将一名无关的健康捐赠者的 FMT 应用于 13 名 IBS 患者。通过 16S 核糖体 RNA 扩增子测序分析患者和捐赠者的粪便样本。结果：在症状水平上，FMT后主要腹痛症状减轻，且未观察到不良反应。通过研究微生物组，我们发现 FMT 后肠道微生物群的 α 多样性增加和组成发生变化。 FMT 后的 7 名患者中，β 多样性变化非常显着，其微生物群谱与捐赠者非常接近。这些患者还表现出最明显的内脏疼痛减轻。在我们的队列中，Akkermansia muciniphila 的相对丰度与疼痛减轻呈负相关。结论：尽管本研究具有探索性和试点性质，但它强调了微生物群控制在 IBS 中的潜在作用，并描述了肠道阿克曼氏菌与疼痛调节的新关联。 (C) 2018 S. Karger AG，巴塞尔
Background/Aims: The gut microbiota is altered in irritable bowel syndrome (IBS), and microbiota manipulations by diet or antibiotics can reduce its symptoms. As fecal microbiota transfer (FMT) in IBS is still controversial, we investigated the clinical and side effects of FMT in a cohort of IBS patients with recurrent, treatment refractory symptoms, and studied gut microbiota signatures. Methods: Using an observational, prospective study design, we applied FMTs from one unrelated, healthy donor to 13 IBS patients. Fecal samples of patients and the donor were analyzed by 16S ribosomal RNA amplicon sequencing. Results: On a symptom level, primarily abdominal pain symptoms were reduced after FMT, and no adverse effects were observed. Studying the microbiome, we found an increase in alpha diversity and changes in the composition of the gut microbiota after FMT. Beta diversity changes after FMT were prominent in a subset of 7 patients with microbiota profiles coming very close to the donor. These patients also showed most pronounced visceral pain reduction. The relative abundance of Akkermansia muciniphila was inversely correlated with pain reduction in our cohort. Conclusion: Although exploratory in nature and with a pilot character, this study highlights the potential role of microbiota manipulations in IBS and describes a novel association of intestinal Akkermansia and pain modulation. (C) 2018 S. Karger AG, Basel