Liposome Flotation Assays for Phosphoinositide-protein Interaction
Liposome Flotation Assays for Phosphoinositide-protein Interaction
复制标题
DOI：
10.21769/bioprotoc.2169
复制
发表时间：
2017-03-05
期刊：
影响因子：
0.8
通讯作者：
Boal, Frederic
中科院分区：
文献类型：
--
作者：
Tronchere, Helene;Boal, Frederic
磷酸肌醇是一种罕见的膜脂质，参与控制主要细胞功能和信号通路。它们能够将特异性效应蛋白募集到质膜和细胞器的胞质表面，以协调各种各样的信号传导和运输过程，以及维持不同亚细胞区室的特异性身份（Di Paolo和De Camilli，2006; Lemmon，2003）。因此，分析这些效应物对不同磷酸肌醇的结合特性和特异性对于理解它们的细胞功能至关重要。该方案描述了一种方法来表征蛋白质与不同的含磷酸肌醇的囊泡的结合。
Phosphoinositides are rare membrane lipids involved in the control of the major cellular functions and signaling pathways. They are able to recruit specific effector proteins to the cytosolic face of plasma membrane and organelles to coordinate a vast variety of signaling and trafficking processes, as well to maintain specific identity of the different subcellular compartments (Di Paolo and De Camilli, 2006; Lemmon, 2003). Therefore, analysis of these effectors' binding properties and specificity towards different phosphoinositides is crucial for the understanding of their cellular functions. This protocol describes a method to characterize the binding of proteins to different phosphoinositide-containing vesicles.