Number of blastocysts biopsied as a predictive indicator to obtain at least one normal/balanced embryo following preimplantation genetic diagnosis with single nucleotide polymorphism microarray in translocation cases.
Number of blastocysts biopsied as a predictive indicator to obtain at least one normal/balanced embryo following preimplantation genetic diagnosis with single nucleotide polymorphism microarray in translocation cases.
复制标题
在易位病例中使用单核苷酸多态性微阵列进行植入前遗传学诊断后，活检的囊胚数量作为获得至少一个正常/平衡胚胎的预测指标
DOI：
10.1007/s10815-016-0831-0
复制
发表时间：
2017-01
影响因子：
3.1
通讯作者：
Xu YW
中科院分区：
文献类型：
--
作者：
Wang YZ;Ding CH;Wang J;Zeng YH;Zhou W;Li R;Zhou CQ;Deng MF;Xu YW
本研究的目的是探讨活检所需的最小囊胚数量，以增加易位携带者在植入前遗传学诊断（PGD）中获得至少一个正常/平衡胚胎的可能性。这项盲法回顾性研究包括 55 个罗伯逊易位 (RT) 周期的 PGD 周期和 181 个相互易位 (rcp) 周期，以表明何时只有一对夫妇携带易位。滋养外胚层活检后进行单核苷酸多态性微阵列。 RT 组中的活检囊胚为 355/379 (93.7%)，rcp 组中的活检囊胚为 986/1053 (93.6%)，获得了可靠的结果。 RT 组中每位患者的平均活检胚胎数、每位患者的正常/平衡胚胎数以及每位患者的平均正常/平衡胚胎率分别为 7.4、3.1 和 40.7%，而 RCP 组分别为 8.0、2.1 和 27.3%。在回归模型中，三个因素显着影响可遗传移植胚胎的数量：活检胚胎数量（P = 0.001）、基础FSH水平（P = 0.040）和母亲年龄（P = 0.027）。以 1.5 为截止值的 ROC 分析计算了为 RT 携带者获得至少一个正常/平衡胚胎所需的活检胚胎数量。对于 rcp 运营商，截止值为 3.5。 RT 组和 rcp 组每次胚胎移植的临床妊娠率分别为 44.2% 和 42.6%（P = 0.836）。在女性年龄 < 37岁且基础FSH水平 < 11.4IU/L的条件下，获得至少一个正常/平衡胚胎进行RT和rcp的囊胚的最小数量为2和4。
The aim of this study is to investigate the minimum number of blastocysts for biopsy to increase the likelihood of obtaining at least one normal/balanced embryo in preimplantation genetic diagnosis (PGD) for translocation carriers. This blinded retrospective study included 55 PGD cycles for Robertsonian translocation (RT) and 181 cycles for reciprocal translocation (rcp) to indicate when only one of the couples carried a translocation. Single-nucleotide polymorphism microarray after trophectoderm biopsy was performed. Reliable results were obtained for 355/379 (93.7 %) biopsied blastocysts in RT group and 986/1053 (93.6 %) in rcp group. Mean numbers of biopsied embryos per patient, normal/balanced embryos per patient, and mean normal/balanced embryo rate per patient were 7.4, 3.1, and 40.7 % in RT group and 8.0, 2.1, and 27.3 %, respectively, in rcp group. In a regression model, three factors significantly affected the number of genetically transferrable embryos: number of biopsied embryos (P = 0.001), basal FSH level (P = 0.040), and maternal age (P = 0.027). ROC analysis with a cutoff of 1.5 was calculated for the number of biopsied embryos required to obtain at least one normal/balanced embryo for RT carriers. For rcp carriers, the cutoff was 3.5. The clinical pregnancy rate per embryo transfer was 44.2 and 42.6 % in RT and rcp groups (P = 0.836). The minimum numbers of blastocysts to obtain at least one normal/balanced embryo for RT and rcp were 2 and 4 under the conditions of female age < 37 years with a basal FSH level < 11.4 IU/L.