Spatial and temporal expression of folate-binding protein 1 (Fbp 1) is closely associated with anterior neural tube closure in mice
Spatial and temporal expression of folate-binding protein 1 (Fbp 1) is closely associated with anterior neural tube closure in mice
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DOI：
10.1002/dvdy.10203
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发表时间：
2003-01-01
影响因子：
2.5
通讯作者：
Shiota, K
中科院分区：
文献类型：
--
作者：
Saitsu, H;Ishibashi, M;Shiota, K
围受孕期补充叶酸被广泛认为对神经管缺陷的产生具有显著的预防作用。叶酸结合蛋白1（Folate-binding protein 1，FBP 1）是介导细胞摄取叶酸的膜蛋白之一。虽然最近的研究表明，Fbp 1是必不可少的神经管关闭，Fbp 1在胚胎组织中的表达模式还没有详细检查。为了阐明Fbp 1如何促进神经管闭合，我们通过原位杂交研究了Fbp 1在小鼠胚胎发育中的神经褶皱和神经管中的时空表达模式。Fbp 1在神经褶皱中表现出独特的表达模式，这在颈部和前脑/中脑边界的神经管闭合开始之前。Fbp 1的表达主要定位于最背侧区域的神经褶皱，融合发生。随着神经褶融合的进行，Fbp 1的表达扩展到相邻的未融合神经褶。在菱脑中，观察到Fbp 1在菱节2（r2）和r6中的稳健表达，表明其在神经嵴细胞发育中的作用。FBP 1在卵黄囊中也有强表达，提示FBP 1可能介导了胚胎神经形成过程中母体叶酸向胚胎的转运。这些发现表明Fbp 1和前神经管关闭之间的密切关联。
Periconceptional folate supplementation is widely believed to have significant preventive effects on the production of neural tube defects. Folate-binding protein 1 (FBP1) is one of the membrane proteins that mediate cellular uptake of folate. Although recent studies suggest that Fbp1 is essential for neural tube closure, the pattern of Fbp1 expression in embryonic tissues has not been examined in detail. To elucidate how Fbp1 contributes to neural tube closure, we examined the spatial and temporal expression patterns of Fbp1 in the developing neural folds and tube of mouse embryos by in situ hybridization. Fbp1 showed a distinct expression pattern in the neural folds, which preceded initiation of neural tube closure at the cervical region and the prosencephalic/mesencephalic boundary. Fbp1 expression was mainly localized to the most dorsal regions of the neural folds where fusion was to occur. With proceeding of neural fold fusion, Fbp1 expression extended to the adjacent unfused neural folds. In the rhombencephalon, robust expression of Fbp1 was observed in rhombomere2 (r2) and r6, suggesting its roles in development of neural crest cells. Fbp1 also showed intense expression in the yolk sac, indicating that FBP1 may mediate transferring maternal folate to embryos during neurulation. These findings indicate close association between Fbp1 and anterior neural tube closure.