Probing Exchange Pathways in One-Dimensional Aggregates with Super-Resolution Microscopy
Probing Exchange Pathways in One-Dimensional Aggregates with Super-Resolution Microscopy
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DOI：
10.1126/science.1250945
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发表时间：
2014-05-02
期刊：
影响因子：
56.9
通讯作者：
Meijer, E. W.
中科院分区：
文献类型：
--
作者：
Albertazzi, Lorenzo;van der Zwaag, Daan;Meijer, E. W.
超分子纤维是生物学和化学中的重要结构。通过超分辨率随机光学重建显微镜和随机模拟相结合，获得了在这些一维聚集体的分子交换途径的定量理解。这种方法的潜力证明了一组定义明确的合成积木，自组装成超分子纤维。以前的合奏测量隐藏所有的分子现象支撑单体交换，但从单聚集体研究确定的分子途径揭示了意想不到的均匀交换沿着聚合物主链。这些结果为合成和天然超分子纤维的结构和交换途径的实验研究奠定了基础。
Supramolecular fibers are prominent structures in biology and chemistry. A quantitative understanding of molecular exchange pathways in these one-dimensional aggregates was obtained by a combination of super-resolution stochastic optical reconstruction microscopy and stochastic simulation. The potential of this methodology is demonstrated with a set of well-defined synthetic building blocks that self-assemble into supramolecular fibrils. Previous ensemble measurements hid all molecular phenomena underpinning monomer exchange, but the molecular pathway determined from single-aggregate studies revealed unexpected homogeneous exchange along the polymer backbone. These results pave the way for experimental investigation of the structure and exchange pathways of synthetic and natural supramolecular fibers.