Purification and Isolation of Proteins from Hyaline Cartilage.
Purification and Isolation of Proteins from Hyaline Cartilage.
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从透明软骨中纯化和分离蛋白质。
DOI：
10.1007/978-1-0716-2839-3_16
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Oxford,JuliaThom
中科院分区：
文献类型：
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作者：
Hardy,MakennaJ;Pu,Xinzhu;Oxford,JuliaThom
来自透明或关节软骨的蛋白质可以使用一系列化学提取步骤和各种鉴定技术（包括质谱法和免疫印迹法）来分离和纯化。从软骨中分离和纯化蛋白质将有助于研究特定的蛋白质和软骨蛋白质的多聚体复合物，以更好地了解它们在正常健康软骨以及软骨病理条件下的功能。软骨组织工程的努力依赖于对软骨的组成和每种蛋白质成分的功能的全面理解。
Proteins from hyaline or articular cartilage can be isolated and purified using a series of chemical extraction steps and various identification techniques including mass spectrometry and immunoblotting. The isolation and purification of proteins from cartilage will facilitate the study of specific proteins and multimeric complexes of cartilage proteins to better understand their functions in normal healthy cartilage as well as pathological conditions of cartilage. Cartilage tissue engineering efforts rely on the comprehensive understanding of the composition of cartilage and the function of each of the protein constituents.