Influence of W-7, a calmodulin antagonist on phospholipid biosynthesis in Candida albicans
Influence of W-7, a calmodulin antagonist on phospholipid biosynthesis in Candida albicans
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DOI：
10.1046/j.1472-765x.2003.01324.x
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发表时间：
2003-01-01
影响因子：
2.4
通讯作者：
Khuller, GK
中科院分区：
文献类型：
--
作者：
Dhillon, NK;Sharma, S;Khuller, GK
目的：本研究利用钙调素拮抗剂W-7研究钙调素在白念珠菌磷脂合成中的作用。在细胞质量，磷脂含量和掺入标记的前体到磷脂和各自的酶的活动的变化已经studied.Results：减少掺入标记的乙酸酯到总脂质和磷脂中观察到的存在下，40妈妈的W-7，这不是作为一个结果的改变生长的念珠菌在钙调素拮抗剂的存在下。此外，钙调蛋白和钙调素依赖性蛋白激酶活性的水平显着下降，观察到在细胞生长与不同浓度的W-7。这是伴随着减少/增加的磷脂酸磷酸酶和磷脂酶A的活性，分别在W-7生长的细胞相比controls.Conclusions：这些研究结果表明，钙调蛋白在白色念珠菌的磷脂代谢的调节明确参与。
Aim: This study was undertaken to investigate the role of calmodulin in phospholipid biosynthesis in Candida albicans using W-7, a calmodulin antagonist.Methods: Cells were grown as shake cultures in the absence and presence of W-7 at different concentrations. Changes in cell mass, phospholipid content and incorporation of labelled precursor into phospholipid and activities of respective enzymes have been studied.Results: Decreased incorporation of labelled acetate into total lipids and phospholipids was observed in the presence of 40 mum of W-7 which was not as a consequence of altered growth of Candida in the presence of calmodulin antagonist. Further, a significant decrease in the levels of calmodulin and CaM dependent protein kinase activity was observed in cells grown with different concentrations of W-7. This was accompanied by decreased/increased activity of phosphatidic acid phosphatase and phospholipase A, respectively in W-7 grown cells as compared to controls.Conclusions: These findings suggest definite involvement of calmodulin in the regulation of phospholipid metabolism in Candida albicans .