NUCLEOTIDE-SEQUENCE OF THE REGION REQUIRED FOR MAINTENANCE OF COLICIN-E1 PLASMID
NUCLEOTIDE-SEQUENCE OF THE REGION REQUIRED FOR MAINTENANCE OF COLICIN-E1 PLASMID
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DOI：
10.1007/bf00273210
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发表时间：
1979-01-01
期刊：
影响因子：
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通讯作者：
TOMIZAWA, JI
中科院分区：
文献类型：
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作者：
OHMORI, H;TOMIZAWA, JI
分离携带不同部分colicin E1质粒（Co1E1） DNA的质粒，以确定细菌中维持质粒所需的Co1E1 DNA区域[大肠杆菌]。为了构建质粒，用限制性内切酶haei切割Co1E1衍生物的DNA，该Co1E1衍生物含有控制氨苄西林耐药性的基因。消化产物通过[噬菌体]T4 DNA连接酶连接，然后转化细菌产生氨苄西林耐药性。以这种方式获得的质粒衍生物通常由某些HaeII片段组成。它们包含大约10%的Co1E1基因组，包括Co1E1复制的起源。Co1E1的区域是所有这些衍生物共同的，是维持质粒所必需的。在描述了这些结果之后，介绍了该区域的核苷酸序列，并讨论了该区域在质粒复制和维持中的可能作用。
Plasmids carrying various portions of colicin E1 plasmid (Co1E1) DNA were isolated to determine the regions of Co1E1 DNA which are required for maintenance of the plasmid in bacteria [Escherichia coli]. To construct the plasmids, the DNA of a Co1E1 derivative that contains a gene which controls ampicillin resistance was cleaved by the restriction endonuclease HaeII. The digestion products were joined by [phage] T4 DNA ligase and then used to transform bacteria to ampicillin resistance. The plasmid derivatives obtained in this way were always composed of certain HaeII segments. These contain approximately 10% of the Co1E1 genome and include the origin of replication of Co1E1. The region of Co1E1 which is common to all these derivatives is required for maintenance of the plasmid. After a description of these results, the nucleotide sequence of this region is presented, and possible roles of the region in plasmid replication and maintenance are discussed.