Effects of elastase-induced emphysema on airway responsiveness to methacholine in rats.
Effects of elastase-induced emphysema on airway responsiveness to methacholine in rats.
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弹性蛋白酶诱导的肺气肿对大鼠气道对乙酰甲胆碱反应性的影响。
DOI：
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发表时间：
1989
影响因子：
3.3
通讯作者：
J. Martin
中科院分区：
文献类型：
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作者：
S. Bellofiore;D. Eidelman;P. Macklem;J. Martin
我们研究了弹性酶诱导的肺气肿对9只雄性褐挪威大鼠肺容量、肺力学和吸入甲胆碱（MCh）后气道反应的影响。5只大鼠在注射弹性蛋白酶前和注射后4周每周进行测量。4只大鼠分别在注射前和注射后3周进行测量；在这些相同的动物中，评估了弹性蛋白酶前后呼气末肺容量变化对气道对MCh反应的影响。通过雾化生理盐水30 s并将MCh浓度从1 mg/ml加倍至64 mg/ml后计算的肺阻力峰值（RL）来确定气道反应。猪胰弹性酶（1 IU/g）气管内给药。弹性蛋白酶前RL由0.20 +/- 0.02 cmh20 .ml-1升高。MCh （64 mg/ml）后，s（平均+/- SE； n = 9）至0.57 +/- 0.06。浓度-响应曲线呈平台型。静态顺应性与RL最大增幅（δ RL64）呈极显著相关（r = 0.799, P < 0.01）。使用弹性蛋白酶后3周，气道对MCh的最大反应增强，未见平稳期；δ RL64为0.78±0.07 cmh20 .ml-1。s，显著高于对照组δ RL64 （0.36 +/- 0.7, P < 0.05）。在使用弹性蛋白酶之前，呼气末肺容量增加到功能残余容量+ 1.56 ml (+/- 0.08 ml)， RL从0.62 +/- 0.05 cmh20 .ml-1显著降低至64 mg MCh/ml。s ~ 0.50±0.03,P < 0.05。（摘要删节250字）
We examined the effects of elastase-induced emphysema on lung volumes, pulmonary mechanics, and airway responses to inhaled methacholine (MCh) of nine male Brown Norway rats. Measurements were made before and weekly for 4 wk after elastase in five rats. In four rats measurements were made before and at 3 wk after elastase; in these same animals the effects of changes in end-expiratory lung volume on the airway responses to MCh were evaluated before and after elastase. Airway responses were determined from peak pulmonary resistance (RL) calculated after 30-s aerosolizations of saline and doubling concentrations of MCh from 1 to 64 mg/ml. Porcine pancreatic elastase (1 IU/g) was administered intratracheally. Before elastase RL rose from 0.20 +/- 0.02 cmH2O.ml-1.s (mean +/- SE; n = 9) to 0.57 +/- 0.06 after MCh (64 mg/ml). A plateau was observed in the concentration-response curve. Static compliance and the maximum increase in RL (delta RL64) were significantly correlated (r = 0.799, P less than 0.01). Three weeks after elastase the maximal airway response to MCh was enhanced and no plateau was observed; delta RL64 was 0.78 +/- 0.07 cmH2O.ml-1.s, significantly higher than control delta RL64 (0.36 +/- 0.7, P less than 0.05). Before elastase, increase of end-expiratory lung volume to functional residual capacity + 1.56 ml (+/- 0.08 ml) significantly reduced RL at 64 mg MCh/ml from 0.62 +/- 0.05 cmH2O.ml-1.s to 0.50 +/- 0.03, P less than 0.05.(ABSTRACT TRUNCATED AT 250 WORDS)