Understanding environmental DNA detection probabilities: A case study using a stream-dwelling char Salvelinus fontinalis
Understanding environmental DNA detection probabilities: A case study using a stream-dwelling char Salvelinus fontinalis
复制标题
DOI：
10.1016/j.biocon.2015.12.023
复制
发表时间：
2016-02-01
影响因子：
5.9
通讯作者：
Schwartz, Michael K.
中科院分区：
文献类型：
--
作者：
Wilcox, Taylor M.;McKelvey, Kevin S.;Schwartz, Michael K.
环境DNA采样(EDNA)已成为检测水生动物的有力工具。先前的研究表明，EDNA方法比传统抽样方法更敏感。然而，影响EDNA检测的因素和由此产生的采样成本仍然不是很清楚。在这里，我们使用多个实验来得出对溪流中溪鱼(Salvelinus Fontinaris)的Edna产生率和下游持久性的独立估计。我们使用这些估计来参数化模型，比较EDNA采样和传统背包电捕鱼的假阴性检测率。我们发现，使用本研究中的方案，Edna在极低的动物密度下具有合理的检测概率(例如，在每条河流公里一条鱼的密度下检测到0.18的概率)，而在种群水平的密度下具有非常高的检测概率(例如，在密度=每100米3条鱼的情况下检测到的概率为0.99)。这比传统的电捕在确定是否有小河鲑鱼方面更为灵敏，在动物稀少的情况下可能会转化为重要的成本节约。我们的发现与越来越多的文献一致，这些文献表明，EDNA采样是检测水生物种的有力工具，特别是那些稀有和难以用传统方法采样的水生物种。(C)2015年提交人。爱思唯尔有限公司出版。
Environmental DNA sampling (eDNA) has emerged as a powerful tool for detecting aquatic animals. Previous research suggests that eDNA methods are substantially more sensitive than traditional sampling. However, the factors influencing eDNA detection and the resulting sampling costs are still not well understood. Here we use multiple experiments to derive independent estimates of eDNA production rates and downstream persistence from brook trout (Salvelinus fontinalis) in streams. We use these estimates to parameterize models comparing the false negative detection rates of eDNA sampling and traditional backpack electrofishing. We find that using the protocols in this study eDNA had reasonable detection probabilities at extremely low animal densities (e.g., probability of detection 0.18 at densities of one fish per stream kilometer) and very high detection probabilities at population-level densities (e.g., probability of detection >0.99 at densities of >= 3 fish per 100 m). This is substantially more sensitive than traditional electrofishing for determining the presence of brook trout and may translate into important cost savings when animals are rare. Our findings are consistent with a growing body of literature showing that eDNA sampling is a powerful tool for the detection of aquatic species, particularly those that are rare and difficult to sample using traditional methods. (C) 2015 The Authors. Published by Elsevier Ltd.