Blood flow restriction exercise stimulates mTORC1 signaling and muscle protein synthesis in older men
Blood flow restriction exercise stimulates mTORC1 signaling and muscle protein synthesis in older men
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DOI：
10.1152/japplphysiol.01266.2009
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发表时间：
2010-05-01
影响因子：
3.3
通讯作者：
Rasmussen, Blake B.
中科院分区：
文献类型：
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作者：
Fry, Christopher S.;Glynn, Erin L.;Rasmussen, Blake B.
Fry CS，Glynn EL，Drummond MJ，Timmerman KL，Fujita S，Abe T，Dhanani S，Volpi E，Rasmussen BB.血流限制运动刺激mTORC 1信号和肌肉蛋白质合成在老年男性。J Appl Physiol 108：1199-1209，2010.首次发表于2010年2月11日; doi：10.1152/japplphysiol.01266.2009。在衰老过程中骨骼肌质量的损失，肌肉减少症，增加了福尔斯和依赖的风险。抗阻运动（RE）是一种有效的康复技术，可以提高肌肉质量和力量;然而，老年人对传统高强度RE刺激肌肉蛋白质合成（MPS）有抵抗力。最近，一种新的康复运动方法，低强度RE，结合血流限制（BFR），已被证明可以刺激哺乳动物雷帕霉素复合物1（mTORC 1）信号转导和MPS在年轻男性。我们假设低强度RE与BFR能够激活mTORC 1信号传导并刺激老年男性的MPS。我们测量了MPS和mTORC 1相关的信号蛋白在7名老年男性（年龄70 +/- 2岁）运动前后。在两种情况下对受试者进行了相同的研究：在BFR运动期间[双侧腿部伸展运动，最大重复次数为1次（1-RM）的20%，压力袖带放置在两条大腿近端，并在200 mmHg下充气]和运动期间不使用压力袖带（Ctrl）。通过稳定同位素技术和免疫印迹法分别在连续的肌肉活检中测定MPS和信号蛋白的磷酸化。BFR运动后MPS较基线增加56%（P < 0.05），而对照组无变化（P > 0.05）。在mTORC 1下游，运动后BFR组的核糖体S6激酶1（S6 K1）磷酸化和核糖体蛋白S6（rpS 6）磷酸化水平升高（P < 0.05）。我们的结论是，低强度RE与BFR相结合，增强mTORC 1信号和MPS在老年男性。BFR运动是一种新的干预措施，可以增强肌肉康复，以抵消肌肉减少症。
Fry CS, Glynn EL, Drummond MJ, Timmerman KL, Fujita S, Abe T, Dhanani S, Volpi E, Rasmussen BB. Blood flow restriction exercise stimulates mTORC1 signaling and muscle protein synthesis in older men. J Appl Physiol 108: 1199-1209, 2010. First published February 11, 2010; doi:10.1152/japplphysiol.01266.2009.-The loss of skeletal muscle mass during aging, sarcopenia, increases the risk for falls and dependence. Resistance exercise (RE) is an effective rehabilitation technique that can improve muscle mass and strength; however, older individuals are resistant to the stimulation of muscle protein synthesis (MPS) with traditional high-intensity RE. Recently, a novel rehabilitation exercise method, low-intensity RE, combined with blood flow restriction (BFR), has been shown to stimulate mammalian target of rapamycin complex 1 (mTORC1) signaling and MPS in young men. We hypothesized that low-intensity RE with BFR would be able to activate mTORC1 signaling and stimulate MPS in older men. We measured MPS and mTORC1-associated signaling proteins in seven older men (age 70 +/- 2 yr) before and after exercise. Subjects were studied identically on two occasions: during BFR exercise [bilateral leg extension exercise at 20% of 1-repetition maximum (1-RM) with pressure cuff placed proximally on both thighs and inflated at 200 mmHg] and during exercise without the pressure cuff (Ctrl). MPS and phosphorylation of signaling proteins were determined on successive muscle biopsies by stable isotopic techniques and immunoblotting, respectively. MPS increased 56% from baseline after BFR exercise (P < 0.05), while no change was observed in the Ctrl group (P > 0.05). Downstream of mTORC1, ribosomal S6 kinase 1 (S6K1) phosphorylation and ribosomal protein S6 (rpS6) phosphorylation increased only in the BFR group after exercise (P < 0.05). We conclude that low-intensity RE in combination with BFR enhances mTORC1 signaling and MPS in older men. BFR exercise is a novel intervention that may enhance muscle rehabilitation to counteract sarcopenia.