Regulation of goblet cell degranulation in isolated pancreatic ducts.
Regulation of goblet cell degranulation in isolated pancreatic ducts.
复制标题
离体胰管中杯状细胞脱粒的调节。
DOI：
10.1152/ajpgi.1995.268.1.g24
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
deOndarza,J
中科院分区：
文献类型：
--
作者：
Hootman,SR;deOndarza,J
使用形态测量程序检查了豚鼠主胰管分离段中杯状细胞脱颗粒的神经体液控制。杯状细胞占主胰管头部上皮细胞群的 25-30%，随着距壶腹部距离的增加，该百分比下降至 5-10%。卡巴胆碱、铃蟾肽和血管活性肠肽（VIP）均刺激导管杯状细胞脱颗粒，但缩胆囊素八肽、促胰液素和组胺则不然。卡巴胆碱对离体胰管杯状细胞脱颗粒的刺激作用被阿托品阻断，并通过同时暴露于 VIP 而增强。这些观察结果表明，豚鼠胰管中的杯状细胞表达铃蟾肽、VIP和毒蕈碱胆碱能受体，并且多种细胞内信号传导途径参与杯状细胞脱颗粒的调节。
Neurohumoral control of goblet cell degranulation in isolated segments of the guinea pig main pancreatic duct was examined using morphometric procedures. Goblet cells represent 25-30% of the epithelial cell population at the head of the main pancreatic duct, a percentage that decreases to 5-10% as the distance from the ampulla increases. Carbachol, bombesin, and vasoactive intestinal peptide (VIP) each stimulated degranulation of duct goblet cells, although cholecystokinin octapeptide, secretin, and histamine did not. The stimulatory effects of carbachol on goblet cell degranulation in isolated pancreatic ducts were blocked by atropine and enhanced by simultaneous exposure to VIP. These observations indicate that goblet cells in guinea pig pancreatic ducts express bombesin, VIP, and muscarinic cholinergic receptors and that multiple intracellular signaling pathways are involved in the regulation of goblet cell degranulation.