THE DIMINUTO GENE OF ARABIDOPSIS IS INVOLVED IN REGULATING CELL ELONGATION
THE DIMINUTO GENE OF ARABIDOPSIS IS INVOLVED IN REGULATING CELL ELONGATION
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DOI：
10.1101/gad.9.1.97
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发表时间：
1995-01-01
影响因子：
10.5
通讯作者：
CHUA, NH
中科院分区：
文献类型：
--
作者：
TAKAHASHI, T;GASCH, A;CHUA, NH
我们从拟南芥T-DNA转化系中分离到一个隐性突变体，命名为diminuto（dim）。在正常生长条件下，该突变体具有非常短的下胚轴、叶柄、茎和根，这是因为沿着这些器官的纵轴沿着的细胞尺寸减小。此外，在黑暗生长的幼苗中，昏暗导致开放的子叶和初生叶的发育。通过T-DNA标签法克隆了DIM基因。DIM编码一种561个氨基酸的新蛋白，其具有核定位信号特征的二分序列结构域。分子和生理研究表明，功能丧失的突变体等位基因不取消幼苗对光或植物激素的反应，虽然光对下胚轴伸长的抑制作用是在突变体中比在野生型更大。此外，dim突变影响β-微管蛋白基因TUB 1的表达，该基因被认为对植物细胞生长很重要。我们的研究结果表明，DIM基因产物在植物细胞伸长的一般过程中起着至关重要的作用。
We have isolated a recessive mutation named diminuto (dim) from T-DNA transformed lines of Arabidopsis thaliana. Under normal growth conditions, the dim mutant has very short hypocotyls, petioles, stems, and roots because of the reduced size of cells along the longitudinal axes of these organs. In addition, dim results in the development of open cotyledons and primary leaves in dark-grown seedlings. The gene for DIM was cloned by T-DNA tagging. DIM encodes a novel protein of 561 amino acids that possesses bipartite sequence domains characteristic of nuclear localization signals. Molecular and physiological studies indicate that the loss-of-function mutant allele does not abolish the response of seedlings to light or phytohormones, although the inhibitory effect of light on hypocotyl elongation is greater in the mutant than in wild type. Moreover, the dim mutation affects the expression of a beta-tubulin gene, TUB1, which is thought to be important for plant cell growth. Our results suggest that the DIM gene product plays a critical role in the general process of plant cell elongation.