Plasmodium falciparum phosphoenolpyruvate carboxykinase is developmentally regulated in gametocytes
Plasmodium falciparum phosphoenolpyruvate carboxykinase is developmentally regulated in gametocytes
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DOI：
10.1016/s0166-6851(00)00191-2
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发表时间：
2000-04-15
影响因子：
1.5
通讯作者：
Hayward, RE
中科院分区：
文献类型：
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作者：
Hayward, RE
疟原虫物种在细胞内发育过程中具有固定二氧化碳的能力。这一过程有助于形成游离氨基酸和代谢物，它们是疟疾寄生虫葡萄糖代谢的最终产物。通过DNA微阵列分析实验，在恶性疟原虫基因组中克隆并鉴定了编码磷酸烯醇式丙酮酸羧激酶(PEPCK)的基因。PEPCK是一种催化CO2固定的酶。PfPEPCK是一种66.2 kDa的ATP依赖性酶，与植物和酵母中的PEPCK有密切的亲缘关系，但与宿主酶人PEPCK明显不同。在寄生虫发育的可传播和合子阶段，相对于无性血液阶段，PfPEPCK转录本抗活性酶水平上调。恶性疟原虫中肠微环境中PfPEPCK在胞外受精卵发育阶段的高表达可能反映了一种葡萄糖稀有的介质，并表明碳水化合物代谢可能切换到糖异生途径。(C)2000 Elsevier Science B.V.保留所有权利。
Plasmodium species have the capacity to fix carbon dioxide during intracellular development. This process contributes to the pool of free amino acids and metabolites, which are the end products of glucose metabolism in the malaria parasite. A gene encoding phosphoenolpyruvate carboxykinase (PEPCK), an enzyme known to catalyze CO2 fixation was identified in the genome of the human parasite Plasmodium falciparum by DNA microarray analysis experiments and was cloned and characterized. PfPEPCK is a 66.2 kDa, ATP-dependent enzyme which is closely related to PEPCK from plants and yeast but markedly different from the host enzyme human PEPCK. PfPEPCK transcript anti active enzyme levels are upregulated in the transmissible and zygote stages of parasite development relative to the asexual blood stages. Elevated expression of PfPEPCK during the extracellular zygote phase of P. falciparum development within the microenvironment of the mosquito midgut may reflect a glucose-rare medium and suggests a possible switch in carbohydrate metabolism to a gluconeogenesis pathway. (C) 2000 Elsevier Science B.V. All rights reserved.