Age-dependent remodelling of ionotropic signalling in cortical astroglia
Age-dependent remodelling of ionotropic signalling in cortical astroglia
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DOI：
10.1111/j.1474-9726.2011.00682.x
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发表时间：
2011-06-01
期刊：
影响因子：
7.8
通讯作者：
Pankratov, Yuriy
中科院分区：
文献类型：
--
作者：
Lalo, Ulyana;Palygin, Oleg;Pankratov, Yuriy
皮质星形胶质细胞表达谷氨酸和ATP的快离子型受体，尽管它们在神经元-胶质细胞通讯中的作用仍有争议。刺激小鼠新皮层中的神经元传入触发由NMDA、P2 X和AMPA受体以及谷氨酸转运体介导的复杂胶质突触电流（GSC）。此外，星形胶质细胞表现出由神经递质的量子释放引起的自发的“微型”GSC。在这里，我们证明了小鼠大脑的成熟和衰老（1至21个月）影响星形胶质细胞中离子型受体的密度及其在GSC生成中的作用。AMPA受体介导的成分在年轻动物中是最大的，并随着年龄的增长而逐渐下降。GSC的P2 X和NMDA成分在年轻小鼠中最小，在成年小鼠（3和6月龄）中最大，并且在老年小鼠中再次降低，可能反映了神经元-胶质细胞回路的重塑。我们的研究结果表明，快速突触之间的神经元和星形胶质细胞在新皮层，可能参与信息处理的神经元-胶质细胞网络在大脑成熟和衰老过程中经历重塑。
P>Cortical astrocytes express fast ionotropic receptors for glutamate and ATP, although their role in neurone-glia communication remains controversial. Stimulation of neuronal afferents in mice neocortex triggers complex glial synaptic currents (GSCs) mediated by NMDA, P2X and AMPA receptors and glutamate transporters. In addition, astrocytes demonstrate spontaneous 'miniature' GSCs resulting from quantal release of neurotransmitters. Here, we demonstrate that maturation and aging of the brain of mice (from 1 to 21 months) affect the density of ionotropic receptors in astrocytes and their role in GSCs generation. The AMPA-receptor-mediated component is the largest in young animals and progressively declines with age. The P2X and NMDA components of GSC are smallest in young, maximal in adult (3 and 6 months old) and once more decrease in old mice, probably reflecting the remodelling of neuronal-glial circuitry. Our results demonstrate that fast synaptic transmission between neurones and astrocytes in neocortex that may be involved in information processing in neuronal-glial networks undergoes remodelling during brain maturation and aging.