HEPATIC CYTOSOLIC GLUTATHIONE-S-TRANSFERASE ACTIVITIES IN AGING BROWN NORWAY RATS - IMPORTANCE OF SEX-DIFFERENCES AND PHENOBARBITAL TREATMENT FOR STUDIES OF AGING
HEPATIC CYTOSOLIC GLUTATHIONE-S-TRANSFERASE ACTIVITIES IN AGING BROWN NORWAY RATS - IMPORTANCE OF SEX-DIFFERENCES AND PHENOBARBITAL TREATMENT FOR STUDIES OF AGING
复制标题
DOI：
10.1016/0047-6374(90)90026-c
复制
发表时间：
1990-08-01
影响因子：
5.3
通讯作者：
ROGIERS, V
中科院分区：
文献类型：
--
作者：
COECKE, S;VANDENBERGHE, Y;ROGIERS, V
在对照条件下和给予苯巴比妥后，研究了两种性别的挪威棕色大鼠（11-44周龄）的肝细胞质谷胱甘肽S-转移酶对1-氯-2,4-二硝基苯和1,2-二氯-4-硝基苯活性与年龄相关的变化。使用这两种底物，在对照大鼠（15-53 周龄）中观察到谷胱甘肽 S-转移酶活性的微小变化。对于这些特定年龄组，男性谷胱甘肽 S-转移酶活性显着高于女性，其中性别相关差异在 1,2-二氯-4-硝基苯结合后最为明显。使用 1-choro-2,4-二硝基苯作为“一般”底物，性别差异从 53 周龄开始趋于减小，到 112 周龄时变得不显着。苯巴比妥给药显着增加了两性的总同工酶 3-3 和 3-4 活性，在最年轻和最年长的动物中分别观察到最高和最低的增加。因此可以得出结论，就对照大鼠和苯巴比妥治疗大鼠的谷胱甘肽S-转移酶活性而言，存在一些与年龄相关的变化，但观察到的变化相当小。相反，发现参数“性别”和“苯巴比妥治疗”是造成观察到的主要活性变化的原因。
Age-associated alterations of hepatic cytosolic glutathione S-transferase activities towards 1-chloro-2,4-dinitrobenzene and 1,2-dichloro-4-nitrobenzene were investigated in Brown Norway rats of both sexes (11-44 weeks old), under control conditions and after administration of phenobarbital. With both substrates, small changes in glutathione S-transferase activities are observed for the control rats (15-53 weeks old). For these specific age groups, male glutathione S-transferase activities are significantly higher than those of their female counterparts, with sex-related differences being most pronounced after 1,2-dichloro-4-nitrobenzene conjugation. Using 1-choro-2,4-dinitrobenzene as a ''general'' substrate, the sex-differences tend to decrease from the age of 53 weeks onwards to become non-significant at the age of 112 weeks. Phenobarbital adminstration significantlly increases the total and the isoenzymes 3-3 and 3-4 activities in both sexes, with the highest and the lowest increase being observed in the youngest and oldest animals, respectively. It therefore can be concluded that some age-related variations exist as far as the glutathione S-transferase activity of both control and phenobarbital-treated rats are concerned, but that the changes observed are rather small. On the contrary, the parameters ''Sex'' and ''Phenobarbital treatment'' are found to be responsible for the major activity changes observed.