A 1H-NMR plasma metabonomic study of acute and chronic stress models of depression in rats
A 1H-NMR plasma metabonomic study of acute and chronic stress models of depression in rats
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大鼠急性和慢性抑郁应激模型的 1H-NMR 血浆代谢组学研究
DOI：
10.1016/j.bbr.2012.11.036
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发表时间：
2013-03-15
影响因子：
2.7
通讯作者：
Qin, Xuemei
中科院分区：
文献类型：
--
作者：
Shi, Biyun;Tian, Junsheng;Qin, Xuemei
为了研究和比较三种基于应激的抑郁症模型的代谢组学特征，研究了急性和慢性应激对全身内源性代谢物产生的影响。这种代谢组学分析可能为研究人员选择合适的动物模型研究抑郁症和抗抑郁药物提供一种新的途径。大鼠采用三种应激模型：CUMS、FST-1d或FST-14d。用核磁共振结合多变量和统计技术分析血浆中内源性代谢产物。代谢组学研究表明，不同代谢产物的浓度可用于区分抑郁模型：TMA、天冬氨酸、谷氨酸、acac、Nac、丙氨酸、乳酸、Leu/Ile、血脂在CUMS模型中升高，而Pro、β-Hb、Valine降低；TMA在FST-1d模型中降低，α-葡萄糖、β-葡萄糖、β-Hb、Valine和血脂在FST-14d模型中升高。结果表明，代谢组学是一种潜在的合适的评估抑郁模型的方法。代谢组学研究表明，CUMS模型比急性FST-1d模型和可预测FST-14d模型更适用、更敏感。CUMS模型更适合于研究抗抑郁药物的疗效及其作用机制，而FST-14d模型应仅用于评估治疗效果。(C)2012爱思唯尔B.V.保留所有权利。
To investigate and compare the metabonomic profiles of three stress-based models of depression, the effects of acute and chronic stress on the production of systemic endogenous metabolites were investigated. Such metabonomic analysis may provide researchers a new way of selecting appropriate animal models for the study of depression and antidepressants. Rats were subjected to one of three stress-based models: CUMS, FST-1d, or FST-14d. Endogenous metabolites excreted in plasma were analyzed using NMR in conjunction with multivariate and statistical techniques. The metabonomic study indicated that the concentration of different plasma metabolites could be used to differentiate among depression models: TMA, aspartic acid, glutamate, AcAc, NAc, alanine, lactate, Leu/Ile, lipids increased and proline, beta-HB, valine decreased in the CUMS model; TMA decreased in the FST-1d model; alpha-glucose, beta-glucose, beta-HB, valine and lipids increased in the FST-14d model. The results suggested that metabonomics is a potentially appropriate method for evaluating depression models. According to the metabonomics study, CUMS model was more suitable and sensitive than the acute FST-1d model and predictable FST-14d model. The CUMS model was more appropriate for investigating both the efficacy of antidepressants and their mechanisms of action, while the FST-14d model should only be used for evaluating the efficacy of treatment. (C) 2012 Elsevier B.V. All rights reserved.