Stable carbon isotopic evidence for differences in the dietary origin of bone cholesterol, collagen and apatite: Implications for their use in palaeodietary reconstruction
Stable carbon isotopic evidence for differences in the dietary origin of bone cholesterol, collagen and apatite: Implications for their use in palaeodietary reconstruction
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DOI：
10.1016/s0016-7037(03)00216-3
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发表时间：
2004-01-01
影响因子：
5
通讯作者：
Evershed, RP
中科院分区：
文献类型：
--
作者：
Jim, S;Ambrose, SH;Evershed, RP
用各种同位素控制的饮食饲养大鼠，所述饮食包含20%C-3、C-4或海洋蛋白质和C-3和/或C-4非蛋白质或能量（即蔗糖、淀粉和油）常量营养素。对从这些动物的饮食（n = 7）和骨（n = 15）中分离的胆固醇进行化合物特异性稳定碳同位素（813）分析，并将该值与骨胶原和磷灰石的本体δ（13）C测量值进行比较。采用线性回归分析，研究了这三种骨生化成分所反映的膳食信号。骨胆固醇的δ（13）C值显示为反映整个饮食δ（13）C值，胶原主要反映饮食蛋白值，磷灰石反映整个饮食值。膳食蛋白质与能量间隔之间的进一步相关性（Delta（13）C（蛋白质-能量）= δ（13）C（蛋白质）-δ（13）C（能量））和全膳食-骨组分分数（Delta（13）C（bcomp-wdiet）= delta（13）C（骨成分）-δ（13）C-（全膳食））表明对于其中蛋白质δ（13）C值等于能量值的假设膳食，全膳食与骨生化组分之间的分数为胆固醇-3.3ppm，胶原+5.4ppm，磷灰石+9.5ppm。此外，在磷灰石-胆固醇间距（δ（13）C（apat-bchol））中观察到的变化范围很窄，表明胆固醇δ（13）C值也可能用作磷灰石δ（13）C值同位素完整性的独立测试。这些见解骨胆固醇，胶原蛋白和磷灰石饮食信号，饮食骨分馏和骨成分骨成分间距提供了更准确的解释考古人口和食物网的饮食行为时，δ（13）C分析骨的基础。版权所有（C）2004 Elsevier Ltd.
Rats were raised on a variety of isotopically controlled diets comprising 20% C-3, C-4 or marine protein and C-3 and/or C-4 non-protein or energy (i.e. sucrose, starch and oil) macronutrients. Compound specific stable carbon isotope (813 Q analysis was performed on the cholesterol isolated from the diet (n = 7) and bone (n = 15) of these animals and the values compared with bulk delta(13)C measurements of bone collagen and apatite. The dietary signals reflected by these three bone biochemical components were investigated using linear regression analysis. delta(13)C values of bone cholesterol were shown to reflect whole diet delta(13)C values, collagen to reflect mainly dietary protein values and apatite to reflect whole diet values. Further correlations between dietary protein-to-energy spacings (Delta(13)C(prot-engy) = delta(13)C(protein) - delta(13)C(energy)) and whole diet-to-bone component fractionations (Delta(13)C(bcomp-wdiet) = delta(13)C(bone component) - delta(13) C-(whole diet)) indicates that for hypothetical diets where protein delta(13)C values are equal to energy values, fractionations between whole diet and bone biochemical fractions are -3.3parts per thousand for cholesterol, +5.4parts per thousand for collagen and +9.5parts per thousand for apatite. Moreover, the narrow range of variation observed in apatite-to-cholesterol spacings (Delta(13)C(apat-bchol)) suggests that cholesterol delta(13)C values can potentially also be used as an independent test for the isotopic integrity of apatite delta(13)C values. These insights into bone cholesterol, collagen and apatite dietary signals, diet-to-bone fractionations and bone component-to-bone component spacings provide the basis for more accurate interpretations of the dietary behaviour of archaeological populations and food webs when the delta(13)C analysis of bone is employed. Copyright (C) 2004 Elsevier Ltd.