Zebrafish genetic map with 2000 microsatellite markers
Zebrafish genetic map with 2000 microsatellite markers
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DOI：
10.1006/geno.1999.5824
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发表时间：
1999-06-15
期刊：
影响因子：
4.4
通讯作者：
Fishman, MC
中科院分区：
文献类型：
--
作者：
Shimoda, N;Knapik, EW;Fishman, MC
斑马鱼是第一个用于大规模遗传筛选的脊椎动物生物体，这些筛选非常成功，发现了1800多个隐性突变，这些突变与脊椎动物胚胎的形态发生有关。突变基因的克隆依赖于密集的遗传图谱。我们在这里提出的2000个标记，使用微卫星（CA）重复，提供1，2-cM的平均分辨率。在斑马鱼中，1厘摩约为0.74兆碱基，因此，对于许多突变，这些标记足够接近，可以通过YAC步移开始定位克隆。(C)北京：科学出版社.
The zebrafish is the first vertebrate organism used for large-scale genetic screens seeking genes critical to development, These screens have been quite successful, with more than 1800 recessive mutations discovered that speak to morphogenesis of the vertebrate embryo. The cloning of the mutant genes depends on a dense genetic map. The 2000 markers we present here, using microsatellite (CA) repeats, provides 1,2-cM average resolution. One centimorgan in zebrafish is about 0.74 megabase, so, for many mutations, these markers are close enough to begin positional cloning by YAC walks. (C) 1999 Academic Press.