Pterygial body epithelium domination of pterygial proliferation with TCF4 as a potential key factor
Pterygial body epithelium domination of pterygial proliferation with TCF4 as a potential key factor
复制标题
翼状胬肉体上皮对翼状胬肉增殖的主导作用，TCF4是潜在的关键因素
DOI：
10.18240/ijo.2018.09.07
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发表时间：
2018-09-18
影响因子：
1.4
通讯作者：
Lu, Rong
中科院分区：
文献类型：
--
作者：
Nie, Cong;Zhang, Xin-Chun;Lu, Rong
目的：为了解翼状胬肉不同部位（头、颈、体）上皮细胞增殖能力，探讨转录因子4（TCF 4）在翼状胬肉增殖中的作用。通过克隆分析、增殖倍数分析和免疫荧光染色及实时荧光PCR检测增殖标志物的表达谱，测定头、颈、体部上皮细胞的增殖能力。TCF 4与增殖细胞核抗原（PCNA）、ATP结合盒亚家族G成员2（ABCG 2）等增殖相关标志物的双重免疫荧光染色结果显示：结膜上皮细胞的增殖能力高于正常结膜上皮细胞。免疫荧光染色和实时荧光定量PCR检测结果显示，P63 α、PCNA和ABCG 2在垂体体上皮细胞中呈高表达（P
AIM: To characterize the proliferative capacity of pterygial epithelium in different regions (head, neck and body) of pterygium and explore the function of transcription factor 4 (TCF4) in pterygium proliferation.METHODS: Thirty pterygium tissues and 10 normal conjunctival tissues were obtained from Zhongshan Ophthalmic Center (ZOC) and Guangdong Eye Bank, respectively. Proliferative capacity of head, neck and body in pterygial epithelium was measured using clonal analysis, fold growth analysis and expression profile of proliferative markers revealed by immunofluorescent staining and real-time PCR. The expression of TCF4 was highlighted by double immunofluorescent staining with other proliferation related markers such as proliferating cell nuclear antigen (PCNA) and ATP-binding cassette subfamily G member 2 (ABCG2).RESULTS: The proliferative potential of pterygial epithelium was higher than that of normal conjunctival epithelium. High expression levels of proliferative markers (P63 alpha, PCNA and ABCG2) in pterygial body epithelium were observed in immunofluorescent staining and real-time PCR (P