A prototype two-dimensional capillary electrophoresis system fabricated in poly(dimethylsiloxane)
A prototype two-dimensional capillary electrophoresis system fabricated in poly(dimethylsiloxane)
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DOI：
10.1021/ac0109422
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发表时间：
2002-04-15
影响因子：
7.4
通讯作者：
Whitesides, GM
中科院分区：
文献类型：
--
作者：
Chen, XX;Wu, HK;Whitesides, GM
提出了一种以毛细管形式进行2D GET电泳的方法。在该方法中，第一维分离在ID毛细管中进行，该系统与提供第二维分离的毛细管物理隔离。在完成第一次分离之后，将1D通道物理地连接到2D毛细管，并在一组正交的平行毛细管中进行第二次分离。聚二甲基硅氧烷(PDMS)支持制造3D微流体系统的能力使其有可能产生膜，既可以将第一次分离中使用的凝胶封闭在毛细管中，又可以为蛋白质迁移到正交毛细管阵列中提供通道。PDMS的弹性性质使得在PDMS之间进行可逆连接成为可能。使用含有荧光素标记的碳酸氢酶、荧光素标记的牛血清白蛋白和德克萨斯红标记的卵清蛋白的蛋白质混合物证明了该系统的可行性。这项工作提出了一种可能形成2D毛细管电泳微制造设备的基础的设计。
A method for carrying out 2D get electrophoresis in a capillary format is presented. In this method, separation in the first dimension is carried out in a ID capillary, with this system physically isolated from the capillaries that provide the separation in the second dimension. After completion of the first separation, the 1D channel is physically connected to the 2D capillaries, and a second separation is carried out in an orthogonal set of parallel capillaries. The ability of poly(dimethylsiloxane) (PDMS) to support the fabrication of 3D microfluidic systems makes it possible to produce membranes that both enclose the gel used in the first separation in a capillary and provide passages for the proteins to migrate into the array of orthogonal capillaries. The elastomeric nature of PDMS makes it possible to make reversible connections between pieces of PDMS. The feasibility of this system is demonstrated using a protein mixture containing fluorescein-conjugated carbonic anhydrase, fluorescein-conjugated BSA, and Texas Red-conjugated ovalbumin. This work suggests one type of design that might form the basis for a microfabricated device for 2D capillary electrophoresis.