CNS sites controlling the gastric pyloric sphincter: Neuroanatomical and functional study in the rat.
CNS sites controlling the gastric pyloric sphincter: Neuroanatomical and functional study in the rat.
复制标题
控制胃幽门括约肌的中枢神经系统部位：大鼠的神经解剖学和功能研究。
DOI：
10.1002/cne.25530
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Sahibzada,Niaz
中科院分区：
文献类型：
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作者：
Richardson,Janell;Dezfuli,Ghazaul;Mangel,AllenW;Gillis,RichardA;Vicini,Stefano;Sahibzada,Niaz
幽门括约肌接受迷走神经背侧运动核（DMV）的副交感迷走神经支配。然而，对其高级神经元和参与控制幽门的DMV神经元的核知之甚少。本研究报告有两个目的。首先，确定高级神经元和DMV之间的神经解剖学联系。这是通过将跨神经元伪狂犬病病毒PRV-152注射到大鼠幽门环中并检查这些动物的脑以进行PRV标记来进行的。第二，确定DMV内功能性控制幽门括约肌运动和张力的特定位点。对于这些研究，进行了实验以评估DMV刺激对氨基甲酸乙酯麻醉雄性大鼠幽门活动的影响。将应变计力传感器缝合到幽门张力上以监测张力和运动性。将L-谷氨酸（500 pmol/30 nL）单侧显微注射到DMV的吻侧和尾侧区域。结果表明，PRV标记的神经元主要分布在DMV、中脑中缝核、中脑Edinger-Westphal核、腹侧被盖区、外侧缰核和弓状核。第二项研究的数据表明，在DMV的头端微量注射L-谷氨酸导致静脉注射阿托品和同侧迷走神经切断术阻断幽门的收缩，而在DMV的尾端注射L-谷氨酸则使幽门松弛。同侧迷走神经切断术可消除这种反应，但静脉注射阿托品或L-NG-硝基精氨酸甲酯（L-NAME）不能消除这种反应。这些发现确定了参与控制幽门括约肌的解剖学和功能性脑神经回路。我们的研究结果还表明，DMV的位点特异性刺激可以通过单独的迷走神经通路不同地影响幽门括约肌的活动。
The pyloric sphincter receives parasympathetic vagal innervation from the dorsal motor nucleus of the vagus (DMV). However, little is known about its higher‐order neurons and the nuclei that engage the DMV neurons controlling the pylorus. The purpose of the present study was twofold. First, to identify neuroanatomical connections between higher‐order neurons and the DMV. This was carried out by using the transneuronal pseudorabies virus PRV‐152 injected into rat pylorus torus and examining the brains of these animals for PRV labeling. Second, to identify the specific sites within the DMV that functionally control the motility and tone of the pyloric sphincter. For these studies, experiments were performed to assess the effect of DMV stimulation on pylorus activity in urethane‐anesthetized male rats. A strain gauge force transducer was sutured onto the pyloric tonus to monitor tone and motility.L‐glutamate (500 pmol/30 nL) was microinjected unilaterally into the rostral and caudal areas of the DMV. Data from the first study indicated that neurons labeled with PRV occurred in the DMV, hindbrain raphe nuclei, midbrain Edinger–Westphal nucleus, ventral tegmental area, lateral habenula, and arcuate nucleus. Data from the second study indicated that microinjectedL‐glutamate into the rostral DMV results in contraction of the pylorus blocked by intravenously administered atropine and ipsilateral vagotomy.L‐glutamate injected into the caudal DMV relaxed the pylorus. This response was abolished by ipsilateral vagotomy but not by intravenously administered atropine or L‐NG‐nitroarginine methyl ester (L‐NAME). These findings identify the anatomical and functional brain neurocircuitry involved in controlling the pyloric sphincter. Our results also show that site‐specific stimulation of the DMV can differentially influence the activity of the pyloric sphincter by separate vagal nerve pathways.