Binding-Directed Energy Transfer of Conjugated Polymer Materials for Dual-Color Imaging of Cell Membrane
Binding-Directed Energy Transfer of Conjugated Polymer Materials for Dual-Color Imaging of Cell Membrane
复制标题
用于细胞膜双色成像的共轭聚合物材料的结合定向能量转移
DOI：
10.1021/acs.chemmater.6b01424
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发表时间：
2016-07-12
影响因子：
8.6
通讯作者：
Wang, Shu
中科院分区：
文献类型：
--
作者：
Cui, Qianling;Wang, Xiaoyu;Wang, Shu
生物分子或探针与细胞膜的结合对于研究细胞形态和各种生物学过程具有重要意义。在此，我们设计了一种水溶性共轭聚合物作为膜探针。该探针对脂膜有很强的亲和力，这是由于丰富的咪唑基团具有较高的电荷密度，以及来自共轭主链的中等刚性和疏水性。当与膜结合时，探针的链间FRET显著增强，从而导致蓝色和红色荧光的发射。这对于双色成像是有利的。最后，细胞实验证明，这种大分子探针具有与细胞膜稳定结合的优异性能，即使在长时间保留后也不会出现细胞内吞现象。
Binding of biomolecules or probes to the plasma membrane is of great importance for investigations of cell morphology and various biological processes. Herein, a water-soluble conjugated polymer is designed as a membrane probe. The probe shows a strong affinity toward lipid membranes owing to the high charge density from abundant imidazolium moieties together with the moderate rigidity and hydrophobicity derived from the conjugated backbone. Upon binding with a membrane, the interchain FRET of the probe was substantially enhanced, which resulted in the emission of both blue and red fluorescence. This is favorable for dual-color imaging. Finally, cellular experiments demonstrate the excellent performance of this macromolecular probe on stable binding with cell membranes without the appearance of cell endocytocysis even after a long retention time.