VIRAL AND HOST CELLULAR TRANSCRIPTION IN AUTOGRAPHA-CALIFORNICA NUCLEAR POLYHEDROSIS VIRUS-INFECTED GYPSY-MOTH CELL-LINES
VIRAL AND HOST CELLULAR TRANSCRIPTION IN AUTOGRAPHA-CALIFORNICA NUCLEAR POLYHEDROSIS VIRUS-INFECTED GYPSY-MOTH CELL-LINES
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DOI：
10.1128/jvi.66.5.2966-2972.1992
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发表时间：
1992-05-01
影响因子：
5.4
通讯作者：
DOUGHERTY, E
中科院分区：
文献类型：
--
作者：
GUZO, D;RATHBURN, H;DOUGHERTY, E
苜蓿银纹夜蛾多囊膜核型多角体病毒（AcMNPV）感染两种舞毒蛾细胞系（IPLB-Ld 652 Y和IPLB-LdFB）的特征在于极度减毒的病毒蛋白合成，随后是所有病毒和细胞蛋白产生的完全停滞。在这项研究中，AcMNPV和宿主细胞特异性转录进行了检查。感染的舞毒蛾细胞中病毒RNA的总体水平在大多数测量时间与来自允许AcMNPV复制的感染细胞系（TN-368）的RNA水平相当。使用病毒和宿主基因特异性探针的北方印迹（RNA）分析显示，感染后主要是正常长度的病毒和细胞特异性转录本。运输病毒RNA从细胞核到细胞质和转录的稳定性在感染的舞毒蛾细胞也出现正常的AcMNPV感染的TN-368细胞的类似参数相比。从舞毒蛾和TN-368细胞中提取的宿主细胞和病毒mRNA在感染后的不同时间和体外翻译产生了相似的宿主和病毒蛋白质谱。用DNA合成抑制剂阿非迪霉素处理感染的舞毒蛾细胞消除了感染的IPLB-LdFB细胞中的总蛋白质合成关闭，但对感染的IPLB-Ld 652 Y细胞中的蛋白质合成抑制没有影响。在感染早期的病毒转录本的翻译和正常的翻译或转录的宿主细胞基因在以后的时间的情况下，明显的选择性块进行了讨论。
Infection of two gypsy moth cell lines (IPLB-Ld652Y and IPLB-LdFB) by the Autographa californica multiple-enveloped nuclear polyhedrosis virus (AcMNPV) is characterized by extremely attenuated viral protein synthesis followed by a total arrest of all viral and cellular protein production. In this study, AcMNPV- and host cell-specific transcription were examined. Overall levels of viral RNAs in infected gypsy moth cells were, at most measured times, comparable to RNA levels from an infected cell line (TN-368) permissive for AcMNPV replication. Northern blot (RNA) analyses using viral and host gene-specific probes revealed predominantly normal-length virus- and cell-specific transcripts postinfection. Transport of viral RNAs from the nucleus to the cytoplasm and transcript stability in infected gypsy moth cells also appeared normal compared with similar parameters for AcMNPV-infected TN-368 cells. Host cellular and viral mRNAs extracted from gypsy moth and TN-368 cells at various times postinfection and translated in vitro yielded similar spectra of host and viral proteins. Treatment of infected gypsy moth cells with the DNA synthesis inhibitor aphidicolin eliminated the total protein synthesis shutoff in infected IPLB-LdFB cells but had no effect on protein synthesis inhibition in infected IPLB-Ld652Y cells. The apparent selective block in the translation of viral transcripts early in infection and the absence of normal translation or transcription of host cellular genes at later times is discussed.