Antimicrobial activity of plant extracts and induction of systemic resistance in tomato plants by mixtures of PGPR strains and Zimmu leaf extract against Alternaria solani
Antimicrobial activity of plant extracts and induction of systemic resistance in tomato plants by mixtures of PGPR strains and Zimmu leaf extract against Alternaria solani
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DOI：
10.1016/j.biocontrol.2009.03.002
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发表时间：
2009-08-01
影响因子：
4.2
通讯作者：
Samiyappan, R.
中科院分区：
文献类型：
--
作者：
Latha, P.;Anand, T.;Samiyappan, R.
测定了20种非寄主植物提取物对番茄早疫病病原菌早疫病链格孢菌（Alternaria solani）菌丝生长的抑制作用。离体研究表明，紫木（Allium cepa L。x Allium sativum L.）对菌丝生长的抑制率最高（87%）。索拉尼在体外和体内试验中，还测试了已知的生物防治剂荧光假单胞菌（Pf 1和Py 15）和枯草芽孢杆菌（Bs 16）单独、一起以及与最有效的植物提取物Zimmu结合用于防治A.索拉尼所有分离物彼此相容，并与Zimmu叶提取物相容。在作为种子处理和叶面施用测试的各种生物制剂中，与其他处理相比，基于滑石的制剂Pf 1 + Py 15 + Bs 16 + Zimmu在降低早疫病发病率方面是上级的。此外，还研究了过氧化物酶（PO）、多酚氧化酶（PPO）、苯丙氨酸解氨酶（PAL）、几丁质酶和β-1，3-葡聚糖酶等防御酶的诱导和酚类物质的积累。与对照相比，Pf 1 + Py 15 + Bs 16 + Zimmu处理的植物中的酶积累更大。本研究揭示了Zimmu基PGPR混剂在提高番茄早疫病抗性中的促生作用和诱导系统抗性作用。(C)2009 Elsevier Inc. All rights reserved.
Extracts from 20 non-host plant species were tested for their ability to inhibit the mycelial growth of Alternaria solani, the causal agent of early blight disease of tomato. In vitro studies indicated that leaf extract of Zimmu (Allium cepa L. x Allium sativum L.) demonstrated the highest inhibition of mycelial growth (87%) of A. solani. Known biocontrol agents Pseudomonas fluorescens (Pf1 and Py15) and Bacillus subtilis (Bs16) were also tested alone, together, and in conjunction with the most effective plant extract, Zimmu, in both in vitro and in vivo experiments for control of A. solani. All isolates were compatible with each other and with Zimmu leaf extract. Among the various bioformulations tested as seed treatment and foliar application, the talc-based formulation of Pf1 + Py15 + Bs16 + Zimmu was superior in reducing the early blight disease incidence when compared to other treatments. Further, induction of defense enzymes, such as peroxidase (PO) and polyphenol oxidase (PPO) phenylalanine ammonia-lyase (PAL), chitinase and beta-1,3-glucanase and accumulation of phenolics were studied. The enzyme accumulation was greater in Pf1 + Py15 + Bs16 + Zimmu treated plants compared to control. The study revealed the probable influence of plant growth promotion and induced systemic resistance (ISR) in enhancing the disease resistance in tomato plants against early blight disease by Zimmu based PGPR mixture. (C) 2009 Elsevier Inc. All rights reserved.