Effect of Predominant Methanogenic Flora on Outcome of Lactose Hydrogen Breath Test in Controls and Irritable Bowel Syndrome Patients of North India
Effect of Predominant Methanogenic Flora on Outcome of Lactose Hydrogen Breath Test in Controls and Irritable Bowel Syndrome Patients of North India
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DOI：
10.1007/s10620-008-0532-3
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发表时间：
2009-07-01
影响因子：
3.1
通讯作者：
Singh, K.
中科院分区：
文献类型：
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作者：
Rana, S. V.;Sinha, S. K.;Singh, K.
产甲烷菌群与氢气（H₂）产生之间的关系被认为是氢气呼气试验（H₂BT）解读中一个可能的混杂因素。因此，本研究进行了前瞻性研究，纳入了154例肠易激综合征（IBS）患者（符合罗马II标准）和286例年龄及性别匹配的明显健康的对照者。每位受试者在禁食过夜后使用25克乳糖进行H₂BT。使用美国Quintron公司的SC Microlyser在基线时以及之后每30分钟测量一次甲烷和H₂，共测量4小时。空腹甲烷浓度<10 ppm的受试者被标记为低甲烷产生者（LMP），>10 ppm的被标记为主要甲烷产生者（PMP）。氢气浓度较基线升高>20 ppm被视为氢气呼气试验阳性。IBS组和对照组分别包括66.78%和67.53%的男性。两组的平均年龄分别为48.52±30.54岁（范围15 - 68岁）和45.67±30.54岁（范围15 - 78岁）。154例IBS患者中有77例（50%）氢气呼气试验阳性，286例对照组中有142例（49.65%）阳性（P>0.05）。还观察到，由于PMP，IBS患者中有5/77（6.49%）氢气呼气试验阴性，对照组中有29/154（20.14%）阴性。PMP影响了6.49% - 20.14%受试者的乳糖氢气呼气试验。与IBS患者相比，这种影响在明显健康的受试者中更为明显。
The relationship between methanogenic flora and hydrogen (H-2) production is considered to be a possible confounding factor in the interpretation of hydrogen breath tests (H2BT). Therefore, the present study was conducted prospectively and included 154 IBS patients (fulfilling Rome II criteria) and 286 age-and-sex-matched apparently healthy controls. Each subject underwent H2BT after overnight fasting using 25 g lactose. Methane and H-2 were measured using an SC Microlyser from Quintron, USA, at baseline and every 30 min for a total of 4 h. Subjects with fasting methane concentration < 10 ppm were labeled as low methane producers (LMP) and > 10 ppm as predominant methane producers (PMP). A rise > 20 ppm over base line in hydrogen concentration was taken as +ve hydrogen breath test. IBS and control groups included 66.78% and 67.53% males, respectively. Mean age in the two groups were 48.52 +/- A 30.54 years (range 15-68 years) and 45.67 +/- A 30.54 years (range 15-78 years), respectively. Hydrogen breath test was +ve in 77/154 (50%) IBS patients and in 142/286 (49.65%) in controls (P > 0.05). It was also observed that the hydrogen breath test was -ve due to PMP in 5/77 (6.49%) of IBS patients and in 29/154 (20.14%) in controls. PMP affected lactose hydrogen breath tests in 6.49-20.14% subjects. This effect is more apparent in apparently healthy subjects as compared to patients with IBS.