Fluorescence Transients in Response to Nutrient Enrichment of Nitrogen- and Phosphorus-limited Microcystis aeruginosa Cultures and Natural Phytoplankton Populations: a Measure of Nutrient Limitation
Fluorescence Transients in Response to Nutrient Enrichment of Nitrogen- and Phosphorus-limited Microcystis aeruginosa Cultures and Natural Phytoplankton Populations: a Measure of Nutrient Limitation
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氮磷限制铜绿微囊藻培养物和天然浮游植物种群营养富集响应的荧光瞬变：营养限制的测量
DOI：
10.1071/pp9950331
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
R. Oliver
中科院分区：
文献类型：
--
作者：
Wood;R. Oliver
本研究介绍了应用叶绿素荧光扰动作为微藻营养限制生物测定的研究结果。监测在不同氮 (N) 和磷 (P) 营养方案下进行化学静态培养的铜绿微囊藻对 NO3-、NH4+ 和 PO43- 加标的可变叶绿素荧光 (ΔF) 响应。在短期（<10 分钟）荧光响应（F）中观察到差异，这与测试生物体的原始营养状态有关。在全营养培养基中呈指数生长的细胞显示 ΔF 没有瞬时变化。向 N 限制细胞中添加 NH4+ 或 NO3- 会导致 ΔF 发生短暂变化，而添加 PO43- 后未观察到任何反应。用 PO43- 或 NH4+ 对 P 限制细胞进行加标会导致 ΔF 发生短暂变化，而对 NO3- 则没有观察到任何反应。因此，可以对培养的铜绿假单胞菌细胞进行简单、快速、营养特异性的营养限制测定。当对来自澳大利亚新南威尔士州马兰比吉河莫德堰池 (34o28'S,144o18'E) 的现场样品进行测试时，检测到明显的营养限制（N 或 P）。利用微藻对营养物加标的荧光反应可以快速、特异性地检测浮游植物培养物和野外种群中的营养限制。
This study presents the results of an investigation of the application of chlorophyll fluorescence perturbations as a bioassay for nutrient limitation in microalgae. The variable chlorophyll fluorescence (ΔF) response of Microcystis aeruginosa, chemostatically cultured under different nitrogen (N) and phosphorus (P) nutrient regimes, to spiking with NO3-, NH4+ and PO43-, was monitored. Differences were observed in the short-term (<10 min) fluorescence response (F) which related to the original nutrient status of the test organism. Cells growing exponentially in full nutrient media showed no transient changes in ΔF. The addition of NH4+ or NO3- to N-limited cells resulted in transient changes to ΔF, while no response was observed following the addition of PO43-. Spiking of P-limited cells with either PO43- or NH4+ resulted in transient changes to ΔF, while no response was observed for NO3-. Thus a simple, rapid, nutrient-specific assay for nutrient limitation was available for cultured cells of M. aeruginosa. When the assay was tested on field samples from the Maude Weir Pool (34o28'S,144o18'E), Murrumbidgee River, NSW, Australia, apparent nutrient limitation, either N or P, was detected. Use of microalgal fluorescence responses to nutrient spiking may allow the rapid and specific detection of nutrient limitation in both cultures and field populations of phytoplankton.