Oxidative Stress Pathways Linked to Apoptosis Induction by Low-Temperature Plasma Jet Activated Media in Bladder Cancer Cells: An In Vitro and In Vivo Study
Oxidative Stress Pathways Linked to Apoptosis Induction by Low-Temperature Plasma Jet Activated Media in Bladder Cancer Cells: An In Vitro and In Vivo Study
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DOI：
10.3390/plasma5020018
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发表时间：
2022-06-01
期刊：
影响因子：
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通讯作者：
Inoue, Keiji
中科院分区：
文献类型：
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作者：
Fukuhara, Hideo;Szili, Endre J.;Inoue, Keiji
非肌肉浸润性膀胱癌术后复发率高，膀胱内灌注治疗后出现间质性肺炎等不良事件，目前治疗方法尚不充分。低温等离子体是一种新的物理治疗形式，它提供了丰富的活性氧（ROS）来源。氧化溶液是在应用于靶细胞之前用血浆预处理水介质产生的，通过氧化应激途径导致癌细胞的破坏。本文研究了血浆活化介质（PAM）在膀胱癌细胞中的作用。PAM处理增加氧化应激，导致细胞周期停滞，并伴随线粒体膜去极化，导致线粒体ROS产生增加。细胞周期阻滞和线粒体ROS生成增加导致caspase 3/细胞色素c活性增加，这可能解释了体外和体内膀胱癌肿瘤诱导凋亡的原因。这些观察结果突出了血浆活化溶液作为一种新的辅助治疗方法在膀胱癌的临床治疗中的潜力。
Current methods used to treat non-muscle invasive bladder cancer are inadequate due to a high recurrence rate after surgery and the occurrence of adverse events such as interstitial pneumonia following intravesical instillation therapy. Low-temperature plasma is a new form of physical therapy that provides a rich source of reactive oxygen species (ROS). Oxidative solutions, created by pre-treatment of aqueous media with plasma before application to target cells, lead to the destruction of cancer cells through oxidative stress pathways. This study focuses on the effects of plasma-activated media (PAM) in bladder cancer cells. PAM treatment increases oxidative stress that leads to cell cycle arrest and concomitantly depolarises the mitochondrial membrane leading to increased mitochondrial ROS production. Cell cycle arrest and increased mitochondrial ROS production led to an increase in caspase 3/cytochrome c activity, which might explain the induction of apoptosis in bladder cancer cells in vitro and in a bladder cancer tumour in vivo. These observations highlight the potential of plasma activated solutions as a new adjuvant therapy in the clinical treatment of bladder cancer.