Pattern-based detection of different proteins using an array of fluorescent protein surface receptors
Pattern-based detection of different proteins using an array of fluorescent protein surface receptors
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DOI：
10.1021/ja039562j
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发表时间：
2004-05-12
影响因子：
15
通讯作者：
Hamilton, AD
中科院分区：
文献类型：
--
作者：
Baldini, L;Wilson, AJ;Hamilton, AD
卟啉衍生物的小型库已被开发为蛋白质“指纹”阵列，其荧光发射在与蛋白质表面结合时发生变化。当与不同的蛋白质一起孵育时，该阵列会产生针对每种蛋白质分析物的特征响应。同时，这种设计可以快速筛选卟啉库，以鉴定高亲和力的蛋白质表面配体。
A small library of porphyrin derivatives whose fluorescence emission changes on binding to protein surfaces has been developed as a protein “fingerprinting” array. When incubated with different proteins, the array yields a characteristic response, specific for every protein analyte. At the same time, this design allows for the rapid screening of the porphyrin library for the identification of high affinity protein surface ligands.