Anti-inflammatory effects of sacran, a novel polysaccharide from Aphanothece sacrum, on 2,4,6-trinitrochlorobenzene-induced allergic dermatitis in vivo
Anti-inflammatory effects of sacran, a novel polysaccharide from Aphanothece sacrum, on 2,4,6-trinitrochlorobenzene-induced allergic dermatitis in vivo
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DOI：
10.1016/j.anai.2011.10.013
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发表时间：
2012-02-01
影响因子：
5.9
通讯作者：
Suganuma, Narufumi
中科院分区：
文献类型：
--
作者：
Ngatu, Nlandu Roger;Okajima, Maiko K.;Suganuma, Narufumi
背景资料：Sacran是从日本九州地区的一条河流中发现的一种藻类--骶骨隐杆藻（Aphanothecesacrum）中提取的一种新的硫酸多糖，目的：研究Sacran对2，4，6-三硝基氯苯（TNCB）诱导的NC/Nga小鼠过敏性皮炎的抑制作用。为了使小鼠致敏，在第1天将150 μ L的5%TNCB应用于每只小鼠的腹部上皮下，并在第8天用15 μ L的1%TNCB应用于小鼠的耳皮肤上进行攻击，然后每隔一天诱导皮肤损伤。测定血清炎症标志物水平，并对耳皮肤标本进行组织病理学检查。另一方面，骶骨的经表皮水分流失进行了评估，在11名志愿妇女干燥skin.Results：表皮应用骶骨在小鼠过敏性皮炎皮损的发展显着抑制和减少抓挠行为发作的次数（P <0.01）。此外，sacran有效抑制IgE（P <0.001）、肿瘤坏死因子α（P = 0.02）、白细胞介素4、白细胞介素5和干扰素γ（P <0.01;与TNCB组中的缓冲液相比）的产生和暴露于化学变应原的耳皮肤中的嗜酸性粒细胞浸润。此外，与外源性透明质酸相比，sacran治疗的人体干燥皮肤志愿者的身体区域显著降低了经表皮水分流失水平（P < .01），这是已知的，以改善皮肤水分和发挥皮肤屏障修复activity.Conclusions：这项研究表明，sacran发挥抗炎作用，通过改善皮肤屏障功能，减少T（H）2细胞因子的产生。(C)2012年美国过敏，哮喘和免疫学学院。由爱思唯尔公司出版All rights reserved.
Background: Sacran is a newly discovered sulfated polysaccharide extracted from an algae, Aphanothece sacrum, grown in a river of the Kyushu region in Japan.Objective: To evaluate sacran's inhibitory effect in 2,4,6-trinitrochlorobenzene (TNCB)-induced allergic dermatitis in NC/Nga mice.Methods: Sacran was extracted by acid and alkaline treatment of A sacrum cyanobacterial biomaterials. To sensitize mice, 150 mu L of 5% TNCB was applied epicutaneously on the abdomen of each mouse on day 1 and challenged with 15 mu L of 1% TNCB applied on the ear skin of mice on day 8 and then every other day to induce skin lesions. Serum levels of inflammatory markers were measured and histopathologic examination of ear skin specimens performed. On the other hand, sacran's transepidermal water loss was evaluated in 11 volunteer women with dry skin.Results: Epicutaneous application of sacran in mice has significantly inhibited the development of allergic dermatitis skin lesions and reduced the number of scratching behavior episodes (P < .01). In addition, sacran efficiently inhibited IgE (P < .001), tumor necrosis factor alpha (P = .02), interleukin 4, interleukin 5, and interferon gamma (P < .01; vs buffer in the TNCB group) production and eosinophilic infiltration in the chemical allergen-exposed ear skin. In addition, sacran-treated body regions of human volunteers with dry skin significantly reduced transepidermal water loss levels compared with exogenous hyaluronic acid (P < .01), which is known to improve skin moisture and exert skin barrier repair activity.Conclusions: This study suggests that sacran exerts anti-inflammatory effects by improving skin barrier function and reducing T(H)2 cytokine production. (C) 2012 American College of Allergy, Asthma & Immunology. Published by Elsevier Inc. All rights reserved.