The parental origin correlates with the karyotype of human embryos developing from tripronuclear zygotes.
The parental origin correlates with the karyotype of human embryos developing from tripronuclear zygotes.
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DOI：
10.5653/cerm.2015.42.1.14
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发表时间：
2015-03
期刊：
影响因子：
--
通讯作者：
Sunde L
中科院分区：
文献类型：
--
作者：
Joergensen MW;Labouriau R;Hindkjaer J;Stougaard M;Kolevraa S;Bolund L;Agerholm IE;Sunde L
以往的研究表明，由卵胞浆内单精子注射(ICSI)受精卵发育而来的三原核(3PN)胚胎具有从三倍体到二倍体的自我纠正机制。3常规体外受精(IVF)后受精卵中也可观察到PN。然而，亲本来源在两种受精方法中是不同的。大多数3PN体外受精受精卵是分散起源的，因此更有可能有两个中心粒，而3PN ICSI受精卵是双性起源的，因此更有可能有一个中心粒。在本研究中，我们检验了3PN胚胎的亲本来源是否与核型相关。每个核的核型是通过四次连续的荧光原位杂交来估计的--每一次都有两个探针--产生了8条不同染色体的定量信息。然后对核型进行比较，并与亲本来源相关。与3PN IVF胚胎相比，3PN ICSI胚胎比3PN IVF胚胎显示出比假设的初始3组染色体更大更协调的减少。3PN体外受精受精卵和3PN ICSI受精卵的亲本来源和中心粒数目的差异可能是核型差异的原因。
It has previously been suggested that embryos developing from intracytoplasmic sperm-injected (ICSI) zygotes with three pronuclei (3PN) are endowed with a mechanism for self-correction of triploidy to diploidy. 3PN are also observed in zygotes after conventional in vitro fertilization (IVF). The parental origin, however, differs between the two fertilization methods. Whereas the vast majority of 3PN IVF zygotes are of dispermic origin and thus more likely to have two centrioles, the 3PN ICSI zygotes are digynic in origin and therefore, more likely to have one centriole. In the present study, we examine whether the parental origin of 3PN embryos correlates with the karyotype. The karyotype of each nucleus was estimated using four sequential fluorescence in situ hybridizations-each with two probes-resulting in quantitative information of 8 different chromosomes. The karyotypes were then compared and correlated to the parental origin. 3PN ICSI embryos displayed a significantly larger and more coordinated reduction from the assumed initial 3 sets of chromosomes than 3PN IVF embryos. The differences in the parental origin-and hence the number of centrioles-between the 3PN IVF and the 3PN ICSI zygotes are likely to be the cause of the differences in karyotypes.