Early Erythrolysis in the Hematoma After Experimental Intracerebral Hemorrhage.
Early Erythrolysis in the Hematoma After Experimental Intracerebral Hemorrhage.
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DOI：
10.1007/s12975-016-0505-3
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发表时间：
2017-04
影响因子：
6.9
通讯作者：
Xi G
中科院分区：
文献类型：
--
作者：
Dang G;Yang Y;Wu G;Hua Y;Keep RF;Xi G
脑出血（ICH）后血凝块中发生红细胞溶解，血红蛋白的释放导致脑损伤，但这种溶解何时发生尚不清楚。本研究检查了大鼠的早期红细胞溶解。ICH大鼠尾状核内注射100 μl自体血，假手术大鼠进行针刺。所有大鼠进行T2和T2* MRI扫描，脑用于组织学和CD 163（血红蛋白清道夫受体）和DARPP-32（神经元标记物）免疫组织化学。在T2* MRI上，血肿内存在明显的异质性，具有高或等信号核心和低信号周边。在相同动物中的苏木精和伊红染色显示核心中显著的红细胞溶解，形成红细胞血影。红细胞溶解的程度与血肿周围神经元丢失的严重程度相关。血肿周围CD 163在ICH后第1天增加，可能参与清除早期溶血引起的血红蛋白。此外，与Wistar京都大鼠相比，ICH导致自发性高血压大鼠更严重的红细胞溶解、神经元丢失和血肿周围CD 163上调。总之，T2*MRI可检测到ICH后凝块中出现早期红细胞溶解，并激活了CD 163。高血压与血肿内红细胞溶解增强有关。
Erythrolysis occurs in the clot after intracerebral hemorrhage (ICH) and the release of hemoglobin causes brain injury but it is unclear when such lysis occurs. The present study examined early erythrolysis in rats. ICH rats had an intra-caudate injection of 100 µl autologous blood and sham rats had a needle insertion. All rats had T2 and T2* MRI scanning and brains were used for histology and CD163 (a hemoglobin scavenger receptor) and DARPP-32 (a neuronal marker) immunohistochemistry. There was marked heterogeneity within the hematoma on T2* MRI, with a hyper- or isointense core and a hypointense periphery. Hematoxylin and eosin staining in the same animals showed significant erythrolysis in the core with the formation of erythrocyte ghosts. The degree of erythrolysis correlated with the severity of perihematomal neuronal loss. Perihematomal CD163 was increased by day 1 after ICH and may be involved in clearing hemoglobin caused by early hemolysis. Furthermore, ICH resulted in more severe erythrolysis, neuronal loss and perihematomal CD163 upregulation in spontaneously hypertensive rats compared to Wistar Kyoto rats. In conclusions, T2*MRI detectable early erythrolysis occurred in the clot after ICH, and activated CD163. Hypertension is associated with enhanced erythrolysis in the hematoma.