ON THE EFFECTS OF HUMAN GALANIN IN MAN
ON THE EFFECTS OF HUMAN GALANIN IN MAN
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DOI：
10.1007/bf00404076
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发表时间：
1993-07-01
期刊：
影响因子：
8.2
通讯作者：
KOFOD, H
中科院分区：
文献类型：
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作者：
HOLST, JJ;BERSANI, M;KOFOD, H
人类甘丙肽最近被分离和测序，发现在几个重要方面与猪甘丙蛋白不同，猪甘丙蛋白迄今用于人类研究。因此，我们合成并纯化了人丙氨酸，并以74 pmol的速率静脉滴注。公斤。六名健康志愿者在高血糖钳夹期间进行60分钟的min-1。钳形是通过静脉滴注葡萄糖来实现的，在对照实验中，葡萄糖滴注的速率已被证明可以将血浆葡萄糖水平维持在12-13 mmol/I 90分钟。在整个输注过程中，丙氨酸浓度达到约1500 pmol/l的平台，而不是输注前和控制水平的20-30 pmol/l。在两个实验中得到的葡萄糖水平是无法区分的。两组患者血浆c肽和胰岛素水平均显著升高，且动态浓度曲线基本一致。血浆胰高血糖素浓度明显下降。然而，在注射丙氨酸期间，生长激素水平增加了8倍。半胱氨酸从血浆中消失，半衰期为3.7±0.4 min，与猪半胱氨酸相似。由此可见，在本研究获得的浓度下，人甘丙肽能有效刺激人体生长激素的分泌，但对胰腺内分泌分泌和葡萄糖代谢无影响。
Human galanin was recently isolated and sequenced and was found to differ from porcine galanin, hitherto used for studies in humans, in several important respects. We therefore synthesized and purified human galanin and infused it i. v. at a rate of 74 pmol . kg-1 . min-1 into six healthy volunteers for 60 min during a hyperglycaemic clamp. The clamp was achieved by i. v. infusion of glucose at a rate which in a control experiment had been demonstrated to maintain the plasma glucose level at 12-13 mmol/I for 90 min. Galanin concentrations reached a plateau of approximately 1500 pmol/l throughout the infusion as opposed to pre-infusion and control levels of 20-30 pmol/l. The glucose levels obtained in the two experiments were indistinguishable. Plasma levels of C-peptide and insulin increased significantly in both experiments and the dynamic concentration curves were almost identical. Glucagon concentrations in plasma decreased significantly and similarly. Growth hormone levels, however, increased eight-fold during galanin infusions. Galanin was eliminated from plasma with a half-life of 3.7 +/- 0.4 min, similar to that of porcine galanin. It is concluded that human galanin powerfully stimulates growth hormone secretion in man, but has no effect on pancreatic endocrine secretion or glucose metabolism in the concentrations obtained in this study.