Immunogenic and protective effects of an oral DNA vaccine against infectious pancreatic necrosis virus in fish
Immunogenic and protective effects of an oral DNA vaccine against infectious pancreatic necrosis virus in fish
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DOI：
10.1016/j.fsi.2009.12.006
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发表时间：
2010-04-01
影响因子：
4.7
通讯作者：
Perez-Prieto, Sara I.
中科院分区：
文献类型：
--
作者：
de las Heras, Ana I.;Rodriguez Saint-Jean, S.;Perez-Prieto, Sara I.
针对传染性胰腺坏死病毒(IPNV)的DNA疫苗和口服DNA免疫疗法在鲑鱼中的研究还很少。在这里，插入了衣壳VP2基因的载体被包裹在海藻酸盐微球中，以避免口服后经历的侵袭性胃肠道疾病。藻酸盐微球能有效地保护编码VP2的PDNA，VP2在免疫鲑鱼的不同器官中早期表达，并持续至少60天。疫苗诱导先天免疫反应，在接种后7天和15天，干扰素的表达比接种空质粒的鱼高10倍以上。同样，干扰素诱导的抗病毒Mx蛋白在免疫后15天达到最高表达，中和抗体也在15天后检测到，尽管它们的滴度在疫苗接种后21天进一步上升。免疫鱼类的保护力很高，在疫苗接种后15天和30天受到攻击时，它们的相对存活率约为80%。与对照组相比，在攻击后45天存活的疫苗鱼中检测到非常低的病毒载量。因此，本研究证明了IPNV-VP2基因在IPNV-DNA疫苗输送中的潜力，以及IPNV-VP2基因与未来构建的质粒的相关性。(C)2009爱思唯尔有限公司。保留所有权利。
DNA vaccines and oral DNA-based immunotherapy against infectious pancreatic necrosis virus (IPNV) have scarcely been studied in salmonid fish. Here, a vector with the capsid VP2 gene inserted was encapsulated in alginate microspheres to avoid the aggressive gastrointestinal conditions experienced following oral administration. Alginate microspheres were effective to protect the pDNA encoding VP2, which was expressed early in different organs of the vaccinated trout and that persisted for at least 60 days. The vaccine induces innate immune responses, raising the expression of IFN more than 10-fold relative to the fish vaccinated with the empty plasmid, at 7 and 15 days post-vaccination. Likewise, maximal expression of the IFN-induced antiviral Mx protein was recorded 15 days post-vaccination and neutralizing antibodies were also detected after 15 days, although their titre rose further at 21 days post-vaccination. Protection was high in the immunized fish, which showed around an 80% relative survival when challenged 15 and 30 days after vaccine delivery. Very low viral load with respect to the control group was detected in the vaccinated fish that survived 45 days after challenge. Thus, this study demonstrates the potential of the encapsulation technique for IPNV-DNA vaccine delivery and the relevance of the IPNV-VP2 gene for future plasmid constructs. (C) 2009 Elsevier Ltd. All rights reserved.