See-through Brains and Diffusion Tensor MRI Clarified Fiber Connections: A Preliminary Microstructural Study in a Mouse with Callosal Agenesis
See-through Brains and Diffusion Tensor MRI Clarified Fiber Connections: A Preliminary Microstructural Study in a Mouse with Callosal Agenesis
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DOI：
10.2463/mrms.2014-0142
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发表时间：
2015-01-01
影响因子：
3
通讯作者：
Arikawa-Hirasawa, Eri
中科院分区：
文献类型：
--
作者：
Kerever, Aurelien;Kamagata, Koji;Arikawa-Hirasawa, Eri
使大脑光学透明的清除方法允许神经网络的高分辨率三维（3D）成像。我们使用扩散张量成像（DTI）和双光子成像的清除大脑分析白色物质在BTBR小鼠。我们证实胼胝体发育不全，并确定了一个异常连合接近第三脑室。DTI和清晰的脑双光子成像显示，这些连合纤维构成了一个前集群的腹侧海马连合，并提供了一个详细的评估小鼠的白色物质结构。
Clearing methods that render the brain optically transparent allow high-resolution three-dimensional (3D) imaging of neural networks. We used diffusion tensor imaging (DTI) and two-photon imaging of cleared brains to analyze white matter in BTBR mice. We confirmed corpus callosum agenesis and identified an abnormal commissure close to the third ventricle. DTI and cleared-brain two-photon imaging revealed that these commissural fibers constituted a frontal clustering of the ventral hippocampal commissure and provided a detailed assessment of white matter structure in mice.