Changing distributions of extracellular matrix components during early wing morphogenesis in Drosophila.
Changing distributions of extracellular matrix components during early wing morphogenesis in Drosophila.
复制标题
果蝇早期翅膀形态发生过程中细胞外基质成分分布的变化。
DOI：
10.1006/dbio.1995.1068
复制
发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Palka,J
中科院分区：
文献类型：
--
作者：
Murray,MA;Fessler,LI;Palka,J
鉴定了一种新的单克隆抗体，其特异性针对果蝇胶原IV分子（基底膜胶原）的羧基末端中的表位。IV型胶原，层粘连蛋白，和额外的细胞外分子，2G 2抗原（2G 2-Ag）的分布，免疫细胞化学在早期翅膀发育。在晚三龄幼虫，胶原蛋白IV和层粘连蛋白包围整个翅盘，而2G 2-Ag仅限于该地区的未来翅袋。在盘外翻后的最初几个小时，所有三种ECM成分都排列在翅袋中所有上皮细胞的基底表面，无论是那些注定要排列在翅静脉中的上皮细胞，还是那些注定要在大的脉间区域中紧密贴壁的上皮细胞。IV型胶原蛋白和层粘连蛋白持续在这些细胞在两个最初的回合的并置的背和腹翼表面;后来，他们成为限制的细胞内衬静脉。2G 2-Ag在蛹期相当早的时候完全消失。IV型胶原似乎至少合成两次，一次是在幼虫中，第二次是在蛹中;在这两次合成之间，IV型胶原被酶裂解，可能被血细胞清除。在一个极端的等位基因birthred的翅膀是气球，形成一个单一的内部空间。IV型胶原蛋白和层粘连蛋白在蛹发育早期的所有基底翅细胞表面都与野生型一样。然而，后来，它们继续排列在突变机翼的整个空腔中，而不是采取限制性分布。在一个完全无脉的翅膀（rhomboidveinletvein），IV型胶原蛋白和层粘连蛋白也普遍存在于早期的基底表面，但完全不存在之间的紧密贴壁翅层后。ECM分布在野生型的翅膀和突变体表明，矩阵中发挥了作用，在建立翅膀的变化模式。一种可能性，加强了最近的研究结果ECM受体在果蝇，是他们参与背腹翼层粘附。我们的研究结果也使我们提出，某些组的功能，区分静脉间细胞可能是在细胞分化过程中连接，从而有助于确定这些细胞表型。这些特征包括细胞骨架特化和某些细胞表面和ECM分子。
A new monoclonal antibody, specific to an epitope in the carboxyl terminus of the Drosophila collagen IV molecule (basement membrane collagen) was identified. The distributions of collagen IV, laminin, and an additional extracellular molecule, the 2G2 antigen (2G2-Ag), were followed immunocytochemically during early wing development. In late third instar larvae, collagen IV and laminin surround the entire wing disc, whereas the 2G2-Ag is limited to the region of the future wing pouch. For the first few hours following eversion of the disc, all three ECM components line the basal surfaces of all epithelial cells in the wing pouch, both those destined to line the wing veins and those destined to become tightly apposed in the large intervein regions. Collagen IV and laminin persist on these cells during the two initial rounds of apposition of dorsal and ventral wing surfaces; later, they become restricted to the cells lining the veins. The 2G2-Ag disappears completely quite early in the pupal period. Collagen IV appears to be synthesized at least twice, once in the larva and a second time in the pupa; in between it is enzymatically cleaved and may be eliminated, probably by hemocytes. In an extreme allele of blistered the wing is ballooned to form a single internal space. Collagen IV and laminin line all basal wing cell surfaces early in pupal development as they do in the wild type. Later, however, they continue to line the entire cavity of the mutant wing rather than assuming a restricted distribution. In a completely veinless wing (rhomboidveinletvein), collagen IV and laminin are also present generally on basal surfaces at early times, but are completely absent between the tightly apposed wing layers later. The ECM distributions both in wild type wings and in mutants suggest that the matrix plays a role in the establishment of the wing variation pattern. One possibility, strengthened by recent findings regarding ECM receptors in Drosophila, is their involvement in dorsal-ventral wing layer adhesion. Our findings also lead us to suggest that certain sets of features which distinguish vein from intervein cells may be linked during cell differentiation and thus help to define these cell phenotypes. The features include cytoskeletal specializations and certain cell surface and ECM molecules.