Monitoring of white-rot fungus during bioremediation of polychlorinated dioxin-contaminated fly ash
Monitoring of white-rot fungus during bioremediation of polychlorinated dioxin-contaminated fly ash
复制标题
多氯二恶英污染飞灰生物修复过程中白腐真菌的监测
DOI：
10.1007/s00253-003-1284-9
复制
发表时间：
2003
影响因子：
5
通讯作者：
R. Kondo
中科院分区：
文献类型：
--
作者：
Hiroto Suhara;C. Daikoku;H. Takata;S. Suzuki;Y. Matsufuji;K. Sakai;R. Kondo
Bioremediation is a low-cost treatment alternative for the cleanup of polychlorinated-dioxin-contaminated soils and fly ash when pollution spread is wide-ranging. An interesting fungus,Ceriporiasp. MZ-340, with a high ability to degrade dioxin, was isolated from white rotten wood of a broadleaf tree from Kyushu Island in Japan. We have attempted to use the fungus for bioremediation of polychlorinated-dioxin-contaminated soil on site. However, we have to consider that this trial has the potential problem of introducing a biohazard to a natural ecosystem if this organism is naturalized. We have therefore developed a monitoring system for the introduced fungus as a part of the examination and evaluation of bioremediation in our laboratory. We have also developed a PCR-based assay to reliably detect the fungus at the bioremediation site. DNA isolated from the site was amplified by PCR using a specific primer derived from internal transcribed spacer region (ITS: ITS1, 5.8S rDNA and ITS2) sequences ofCeriporiasp. MZ-340. We successfully monitoredCeriporiasp. MZ-340 down to 100 fg/µl DNA and down to 2 mg/g mycelium. We also successfully monitored the fungus specifically at the bioremediation site. The polychlorinated dibenzo-p-dioxin and polychlorinated dibenzofuran content was observed to decrease in response to treatment with the fungus. The species-specific PCR technique developed in the present work is useful in evaluating the possibility of on-site bioremediation using the fungusCeriporiasp. MZ-340.
