Application of In-Fusion™ Cloning for the Parallel Construction of E. coli Expression Vectors
Application of In-Fusion™ Cloning for the Parallel Construction of E. coli Expression Vectors
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DOI：
10.1007/978-1-62703-764-8_15
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发表时间：
2014-01-01
期刊：
影响因子：
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通讯作者：
Owens, Raymond J.
中科院分区：
文献类型：
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作者：
Bird, Louise E.;Rada, Heather;Owens, Raymond J.
In-Fusion(TM)克隆是一种不依赖DNA连接酶的灵活克隆技术，在分子生物学中有着广泛的应用。在本章中，我们描述了OPPF-UK中使用In-Fusion(TM)设计和构建表达载体所使用的协议。我们还概述了我们在大肠杆菌中筛选In-Fusion(TM)产生的构建体的小规模表达的方法。
In-Fusion (TM) cloning is a flexible DNA ligase-independent cloning technology that has wide-ranging uses in molecular biology. In this chapter we describe the protocols used in the OPPF-UK to design and construct expression vectors using In-Fusion (TM). Our method for small scale expression screening in Escherichia coli of constructs generated by In-Fusion (TM) is also outlined.