Radical scavenging and antioxidant activity of tannic acid
Radical scavenging and antioxidant activity of tannic acid
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DOI：
10.1016/j.arabjc.2009.12.008
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发表时间：
2010-01-01
影响因子：
6
通讯作者：
Aboul-Enein, Hassan Y.
中科院分区：
文献类型：
--
作者：
Gulcin, Ilhami;Huyut, Zubeyr;Aboul-Enein, Hassan Y.
单宁酸是一种天然存在的植物多酚，由中心葡萄糖分子在其羟基处衍生出一个或多个没食子酰基残基组成。本文采用1，1-二苯基-2-苦肼基自由基（1，1-diphenyl-2-picryl-hydrazyl free radical，1，1-diphenyl-2-picryl-hydrazyl-free radical，1，1-di（DPPH中心点）清除，2，2 '-连氮双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）自由基清除活性，通过硫氰酸铁测定的总抗氧化活性，Fe ~（3+）-Fe ~（2+）转化法测定总还原能力，核黄素-蛋氨酸-光照体系测定超氧阴离子自由基清除能力，过氧化氢清除能力和亚铁离子（Fe ~（2+））螯合能力。此外，丁基羟基茴香醚（BHA），丁基羟基甲苯（BHT），α-生育酚和trolox，生育酚的水溶性类似物，被用作参考抗氧化剂自由基清除剂化合物。单宁酸抑制97.7%的脂质过氧化亚油酸乳剂在15 μ g/mL的浓度。另一方面，上述标准抗氧化剂在45 μ g/mL浓度下对亚油酸乳剂的过氧化反应的抑制率分别为92.2%、99.6%、84.6%和95.6%。此外，鞣酸有一个有效的DPPH？清除能力、ABTS（中心点+）自由基清除能力、超氧阴离子自由基清除能力、过氧化氢清除能力、Fe 3+还原能力和对亚铁离子的金属螯合能力。此外，将这些各种抗氧化剂活性与作为参考抗氧化剂化合物的BHA、BHT、α-生育酚和trolox进行比较。目前的研究表明，单宁酸是一种有效的天然抗氧化成分，可用作食品防腐剂或营养保健品。(C)2009年沙特国王大学。All rights reserved.
Tannic acid, a naturally occurring plant polyphenol, is composed of a central glucose molecule derivatized at its hydroxyl groups with one or more galloyl residues. In the present paper, we examines the in vitro radical scavenging and antioxidant capacity of tannic acid by using different in vitro analytical methodologies such as 1,1-diphenyl-2-picryl-hydrazyl free radical (DPPH center dot) scavenging, 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radical scavenging activity, total antioxidant activity determination by ferric thiocyanate, total reducing ability determination using by Fe3+-Fe2+ transformation method, superoxide anion radical scavenging by riboflavin-methionine-illuminate system, hydrogen peroxide scavenging and ferrous ions (Fe2+) chelating activities. Also, butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), alpha-tocopherol and trolox, a water-soluble analogue of tocopherol, were used as the reference antioxidant radical scavenger compounds.Tannic acid inhibited 97.7% lipid peroxidation of linoleic acid emulsion at 15 mu g/mL concentration. On the other hand, the above mentioned standard antioxidants indicated an inhibition of 92.2%, 99.6%, 84.6% and 95.6% on peroxidation of linoleic acid emulsion at 45 mu g/mL concentration, respectively. In addition, tannic acid had an effective DPPH? scavenging, ABTS(center dot+) radical scavenging, superoxide anion radical scavenging, hydrogen peroxide scavenging, Fe3+ reducing power and metal chelating on ferrous ions activities. Also, those various antioxidant activities were compared to BHA, BHT, alpha-tocopherol and trolox as references antioxidant compounds. The present study shows that tannic acid is the effective natural antioxidant component that can be used as food preservative agents or nutraceuticals. (C) 2009 King Saud University. All rights reserved.