Comparative genomics of Aeromonas veronii: Identification of a pathotype impacting aquaculture globally
Comparative genomics of Aeromonas veronii: Identification of a pathotype impacting aquaculture globally
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DOI：
10.1371/journal.pone.0221018
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发表时间：
2019-08-29
期刊：
影响因子：
3.7
通讯作者：
Lawrence, Mark L.
中科院分区：
文献类型：
--
作者：
Tekedar, Hasan C.;Kumru, Salih;Lawrence, Mark L.
Veronii气单胞菌是一种在水生环境中大量存在的革兰氏阴性菌，可引起人类以及陆生和水生动物的疾病。在本研究中，41个公开可用的A.比较了Veronii的基因组，以研究推定的毒力基因的分布、致病型的全球传播和潜在的毒力机制。A.包括来自美国东南部农场饲养的鲶鱼中运动性气单胞菌败血症爆发的veronii菌株ML 09 -123。传播A。在分散的地理位置发现了Veronii菌株类型。分离株ML 09 -123与中国分离株TH 0426高度相似，表明这两个菌株具有共同的起源，可能代表影响两国水产养殖的致病型。实验证实菌株ML 09 -123对鲶鱼的毒力呈剂量依赖性。子系统类别分布显示，大多数基因组显示出相似的基因组元件分布。Ⅰ型分泌系统（T1 SS）、Ⅱ型分泌系统（T2 SS）、4型菌毛（T4 P）和鞭毛核心元件在所有的A. veronii基因组中，而III型分泌系统（T3 SS），V型分泌系统（T5 SS），VI型分泌系统（T6 SS）和紧密粘附（T3 SS）系统表现出可变的分散。分布的移动的元素是依赖于主机和地理起源，这表明该物种已经经历了相当大的遗传交换。这里提供的数据有助于深入了解A. veronii，并确定了影响全球水产养殖的致病型。
Aeromonas veronii is a gram-negative species abundant in aquatic environments that causes disease in humans as well as terrestrial and aquatic animals. In the current study, 41 publicly available A. veronii genomes were compared to investigate distribution of putative virulence genes, global dissemination of pathotypes, and potential mechanisms of virulence. The complete genome of A. veronii strain ML09-123 from an outbreak of motile aeromonas septicemia in farm-raised catfish in the southeastern United States was included. Dissemination of A. veronii strain types was discovered in dispersed geographical locations. Isolate ML09-123 is highly similar to Chinese isolate TH0426, suggesting the two strains have a common origin and may represent a pathotype impacting aquaculture in both countries. Virulence of strain ML09-123 in catfish in a dose-dependent manner was confirmed experimentally. Subsystem category disposition showed the majority of genomes exhibit similar distribution of genomic elements. The type I secretion system (T1SS), type II secretion system (T2SS), type 4 pilus (T4P), and flagellum core elements are conserved in all A. veronii genomes, whereas the type III secretion system (T3SS), type V secretion system (T5SS), type VI secretion system (T6SS), and tight adherence (TAD) system demonstrate variable dispersal. Distribution of mobile elements is dependent on host and geographic origin, suggesting this species has undergone considerable genetic exchange. The data presented here lends insight into the genomic variation of A. veronii and identifies a pathotype impacting aquaculture globally.