KLF4 translation level is associated with differentiation stage of different pediatric leukemias in both cell lines and primary samples
KLF4 translation level is associated with differentiation stage of different pediatric leukemias in both cell lines and primary samples
复制标题
DOI：
10.1007/s10238-012-0187-4
复制
发表时间：
2013-05-01
影响因子：
4.6
通讯作者：
Tang, Yongmin
中科院分区：
文献类型：
--
作者：
Guo, Xiaoping;Tang, Yongmin
恶性细胞上的生物标志物可能具有预后意义。Kruppel样因子4（KLF 4）在白血病细胞中表达，但其在不同分化阶段白血病中的表达模式及其与临床指标的关系尚不清楚。为探讨KLF 4在白血病中的表达及其临床意义，采用RT-PCR和real-time PCR方法检测了9株白血病细胞系和96例儿童白血病患者KLF 4的表达。KLF 4 mRNA在AML组和ALL组中的表达率分别为31.25%（5/16）和39.29%（11/28），明显低于正常对照组（81.82%（9/11），P < 0.05）。KLF 4 mRNA在ALL（27/52）中的表达明显低于AML（25/52）（P = 0.019），AML-M1与其他亚型间表达差异有统计学意义（P = 0.001），C-ALL与Pre-B ALL间表达差异有统计学意义（P = 0.004），Pro-T ALL与T-ALL间表达差异有统计学意义（P = 0.048）。对白血病细胞系的观察也显示了类似的模式。KLF 4 mRNA相对表达量与CD 34阳性率呈负相关（r =-0.296，P = 0.037），与骨髓原始细胞百分率（r =-0.222，P = 0.137）和白细胞计数（r =-0.058，P = 0.679）无关。KLF 4 mRNA表达水平与总生存期无关（r =-0.063，P = 0.670），KLF 4（较低）组和KLF 4（较高）组的中位生存时间相当（28个月vs. 25个月，P = 0.265）。KLF 4 mRNA表达水平在染色体核型正常与异常白血病组间无显著性差异（P = 0.180），在分子细胞遗传学正常与异常白血病组间亦无显著性差异（P = 0.591）。我们的结论是，KLF 4的翻译水平与不同的白血病的分化阶段，是独立的危险分层的其他参数。
Biomarkers on malignant cells may confer prognostic significance. Kruppel-like factor 4 (KLF4) has been reported to be expressed variably on leukemia cells, but its expression patterns in leukemias with different differentiation stages and its relationships with clinical parameters remain to be elucidated. To examine the KLF4 expression pattern in human leukemias and its clinical significance, RT-PCR and real-time PCR were used to detect KLF4 expression in 9 leukemia cell lines and 96 pediatric leukemia patients. KLF4 mRNA expressed in 5/16 (31.25 %) of AML patients and 11/28 (39.29 %) ALL patients, significantly lower than that in control (9/11, 81.82 %, P < 0.05). The expressions of KLF4 mRNA were much lower in ALL (27/52) compared to AML (25/52) (P = 0.019), AML-M1 to other subtypes of AML (P = 0.001), C-ALL to Pre-B ALL (P = 0.004), Pro-T ALL to T-ALL (P = 0.048). Observation on leukemia cell lines showed the similar pattern. The relative expression of KLF4 mRNA was inversely associated with CD34-positive rates (r = -0.296, P = 0.037), but it was not associated with the blasts percentages in bone marrow (r = -0.222, P = 0.137) and the WBC counts (r = -0.058, P = 0.679). KLF4 mRNA expression level was not related to the overall survival (r = -0.063, P = 0.670), and the median survival times for the KLF4 (Lower) and KLF4 (Higher) groups were comparable (28 vs. 25 months, P = 0.265). Furthermore, no difference was found in KLF4 mRNA expression levels whether in leukemias with normal and abnormal karyotypes (P = 0.180), or in leukemias with normal and abnormal molecular cytogenetics (P = 0.591). We conclude that KLF4 translation level is associated with the differentiation stage of different leukemias and is independent of other parameters of risk stratification.