Risk of transmission of imipenem-resistant Pseudomonas aeruginosa through use of mobile bathing service
Risk of transmission of imipenem-resistant Pseudomonas aeruginosa through use of mobile bathing service
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DOI：
10.1007/bf02898205
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发表时间：
2006-01-01
影响因子：
4.7
通讯作者：
Koike, Kazuko A.
中科院分区：
文献类型：
--
作者：
Sakurai-Komada, Naomi;Hirano, Masako;Koike, Kazuko A.
目的：日本社会对移动的洗浴服务（MBS）的需求正在增加。因此，我们评估了MBS客户和他们的照顾者MBS相关感染的风险，通过检查MBS设备和器具的细菌定植：通过邮票琼脂培养法收集的细菌分离株进行了检查，通过纸片扩散试验，其敏感性以下药物：亚胺培南，环丙沙星，阿米卡星，氨曲南，头孢他啶，美罗培南，哌拉西林，妥布霉素，氧氟沙星和头孢哌酮。结果：从不同采样点共分离到54株铜绿假单胞菌，其中26株（47.3%）来自枕头。18株（33.3%）对亚胺培南（IPM）耐药。17株菌的最低抑菌浓度（MIC）在16 ~ 32 μ g/ml之间，1株菌的MIC大于32 μ g/ml。SpeI-PFGE分型结果显示，18株分离株中有13株亲缘关系较近（F=1.0-0.87）。结论：MBS设备和用具，尤其是枕头，是细菌污染和传播的主要来源，MBS患者及其照顾者存在MBS介导的感染风险。
Objectives: The demand for mobile bathing service (MBS) is increasing in the Japanese society. Therefore, we assessed the risk of MBS-associated infection in MBS clients and their caregivers by examining the bacterial colonization of MBS equipment and utensils.Methods: Bacterial isolates collected by the stamp agar culture method were examined by disk diffusion assay for their susceptibility to the following drugs: imipenem, ciprofloxacin, amikacin, azutreonam, ceftazidim, meropenem, piperacillin, tobramycin, ofloxacin and cefoperazone. Furthermore, these isolates were subtyped by SpeI-pulsed field gel electrophoresis (SpeI-PFGE).Results: Fifty-four P aeruginosa isolates were recovered from different sampling sites, and of these, 26 (47.3%) were isolated from pillows. Eighteen isolates (33.3%) were imipenem (IPM) resistant. The minimum inhibitory concentrations (MICs) of 17 isolates were between 16 and 32 mu g/ml, and the MIC of one isolate was greater than 32 mu g/ml. The SpeI-PFGE typing of IPM-resistant isolates revealed that 13 of the 18 isolates were closely related (F=1.0-0.87).Conclusion: Our findings suggest that MBS equipment and utensils, particularly pillows, are the primary sources of bacterial contamination and transmission and that there is a risk of MBS-mediated infection among MBS clients and their caregivers.