Expression of cFABP and PPAR in trophoblast cells: effect of PPAR ligands on linoleic acid uptake and differentiation
Expression of cFABP and PPAR in trophoblast cells: effect of PPAR ligands on linoleic acid uptake and differentiation
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DOI：
10.1016/j.bbalip.2004.11.017
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发表时间：
2005-02-21
影响因子：
4.8
通讯作者：
Lafond, J
中科院分区：
文献类型：
--
作者：
Daoud, G;Simoneau, L;Lafond, J
在整个妊娠过程中，胎儿的生长部分取决于母体必需脂肪酸（EFA）和长链多不饱和脂肪酸的胎盘转移。所有脂肪酸 (FA) 都可以通过简单扩散穿过脂质双层，例如合体滋养层细胞（多核、终末分化的滋养层细胞）中的脂肪酸。滋养层分化过程伴随着人绒毛膜促性腺激素 (hCG) 分泌的增加和人 Achaete-Scute Homologue-2 (Hash-2) 表达的抑制。此外，在哺乳动物细胞的膜和细胞质中发现了许多 FA 结合蛋白 (FABP)，它们被认为有助于 FA 跨膜转移及其细胞内通道。因此，本研究的目的是根据分化情况研究 cFABP 对人类滋养层细胞摄取亚油酸 (LA) 的影响。此外，由于过氧化物酶体增殖物激活受体（PPAR）调节cFABP的表达并在滋养层细胞分化中发挥重要作用，因此验证了PPAR配体对cFABP表达和分化的影响。在此，我们报道了滋养层细胞分化时肝脏和心脏 FABP（L-和 H-FABP）表达的增加，PPARα和β的抑制，而 PPARγ 水平保持不变。非选择性过氧化物酶体增殖剂苯扎贝特和 LA 会损害滋养层分化，并减少 L-和 H-FABP 表达。此外，钴是一种模拟缺氧的化学物质，可抑制滋养层细胞分化并减少 H-、L-FABP 和 PPAR 的表达。最后，两种治疗均未显示对滋养层细胞摄取 LA 的影响。总之，本研究表明，H-和L-FABP的表达与滋养层细胞对LA的摄取之间不存在相关性，并且苯扎贝特和LA极大地损害了滋养层细胞的分化。 (C) 2004 Elsevier B.V. 保留所有权利。
Throughout gestation, fetal growth depends, in part, on placental transfer of maternal essential fatty acid (EFA) and long-chain polyunsaturated fatty acid. All fatty acid (FA) can cross lipid bilayer by simple diffusion, such as those in the syncytiotrophoblasts, the multinucleated, terminally differentiated trophoblast cells. The trophoblasts differentiation process is accompanied by an increase of human chorionic gonadotropin (hCG) secretion and an inhibition of Human Achaete-Scute Homologue-2 expression (Hash-2). Furthermore, a number of FA-binding proteins (FABPs) have been identified in membrane and cytoplasm of mammalian cells, which are thought to facilitate the transfer of FA across membranes and their intracellular channeling. Thus, the aim of this study was to investigate the implication of cFABPs in linoleic acid (LA) uptake by human trophoblast cells according to differentiation. Moreover, since peroxisome proliferator-activated receptor (PPARs) regulate the expression of cFABP and play an important role in trophoblast cells differentiation, the effects of PPARs ligands are verified on cFABP expression and differentiation. Herein, we reported the increase of the expression of liver and heart FABP (L- and H-FABP) upon differentiation of trophoblast cells, an inhibition of PPARalpha and beta, while PPARgamma levels remains unchanged. The nonselective peroxisome-proliferating agents, bezafibrate and LA, impaired trophoblast differentiation, and reduced L- and H-FABP expression. Furthermore, cobalt, a chemical agent known to mimic hypoxia, inhibits trophoblast cells differentiation and diminishes H-, L-FABP and PPARs expression. Finally, both treatments show no influence on LA uptake by trophoblast cells. In conclusion, this study showed that there is no correlation between the expression of H- and L-FABP and LA uptake by trophoblast cells and that bezafibrate and LA greatly impaired trophoblast cells differentiation. (C) 2004 Elsevier B.V. All rights reserved.