Recombination of transfected DNAs in vertebrate cells in culture.
Recombination of transfected DNAs in vertebrate cells in culture.
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培养的脊椎动物细胞中转染的 DNA 的重组。
DOI：
10.1073/pnas.81.11.3476
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发表时间：
1984
影响因子：
11.1
通讯作者：
Temin,HM
中科院分区：
文献类型：
--
作者：
Bandyopadhyay,PK;Watanabe,S;Temin,HM
我们研究了鸡胚成纤维细胞中转染的非感染性逆转录病毒DNA分子之间的同源和非法重组的频率。重组的频率取决于感染性病毒的形成，并随着重组位点处DNA分子之间的同源性程度而变化，但只有当该区域周围存在同源性区域时才如此。虽然同源重组导致野生型病毒的形成，但非法重组导致在重组位点发生改变的感染性病毒的形成。在D17狗细胞中，转染的DNA和染色体DNA之间也观察到明显的同源重组。
We studied the frequency of homologous and illegitimate recombinations between transfected noninfectious retroviral DNA molecules in chicken embryo fibroblasts. The frequency of recombination was determined by the formation of infectious virus and varied with the extent of homology between the DNA molecules at the site of recombination, but only when there were regions of homology surrounding this region. While homologous recombination led to the formation of wild-type virus, illegitimate recombination resulted in formation of infectious virus with alterations at the site of recombination. Apparent homologous recombination was also observed between transfected and chromosomal DNAs in D17 dog cells.