Visualization of plastid movement in the pollen tube of Arabidopsis thaliana
Visualization of plastid movement in the pollen tube of Arabidopsis thaliana
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DOI：
10.4161/psb.7.1.18484
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发表时间：
2012-01-01
影响因子：
2.9
通讯作者：
Itoh, Ryuuichi D.
中科院分区：
文献类型：
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作者：
Fujiwara, Makoto T.;Yoshioka, Yasushi;Itoh, Ryuuichi D.
植物雄配子体细胞器的动态变化在花粉管生长和细胞质遗传中具有重要意义，因而受到广泛关注。最近，我们揭示了在生活拟南芥花粉粒和管质体的动态行为，使用固有的启动子驱动的FtsZ 1-绿色荧光蛋白（GFP）融合。在这里，我们进一步监测了运动的花粉管质体与肌动蛋白1启动子驱动，基质靶向黄色荧光蛋白（YFP）。在伸长的花粉管中，大多数质体定位于管柄，在那里它们要么表现出迟缓和不稳定的运动，要么表现出快速、定向和长距离的运动，沿着管极性。高效的质体追踪进一步揭示了一群尖端向前的质体，它们在向后移动之前经历了波动运动。YFP标记的质体在花粉中的行为基本上类似于FtsZ 1-GFP标记的质体，从而验证了FtsZ 1-GFP用于同时可视化基质和质体分裂FtsZ环的用途。
Organelle dynamics in the plant male gametophyte has received attention for its importance in pollen tube growth and cytoplasmic inheritance. We recently revealed the dynamic behaviors of plastids in living Arabidopsis pollen grains and tubes, using an inherent promoter-driven FtsZ1-green fluorescent protein (GFP) fusion. Here, we further monitored the movement of pollen tube plastids with an actin1 promoter-driven, stroma-targeted yellow fluorescent protein (YFP). In elongating pollen tubes, most plastids localized to the tube shank, where they displayed either retarded and unsteady motion, or fast, directional, and long-distance movement along the tube polarity. Efficient plastid tracking further revealed a population of tip-forwarding plastids that undergo a fluctuating motion(s) before traveling backward. The behavior of YFP-labeled plastids in pollen basically resembled that of FtsZ1-GFP-labeled plastids, thus validating the use of FtsZ1-GFP for simultaneous visualization of the stroma and the plastid-dividing FtsZ ring.