EXPRESSION OF AROMATASE CYTOCHROME-P-450 IN PREMENOPAUSAL AND POSTMENOPAUSAL HUMAN OVARIES - AN IMMUNOCYTOCHEMICAL STUDY
EXPRESSION OF AROMATASE CYTOCHROME-P-450 IN PREMENOPAUSAL AND POSTMENOPAUSAL HUMAN OVARIES - AN IMMUNOCYTOCHEMICAL STUDY
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DOI：
10.1210/jcem-73-4-717
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发表时间：
1991-10-01
影响因子：
5.8
通讯作者：
BRODIE, AMH
中科院分区：
文献类型：
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作者：
INKSTER, SE;BRODIE, AMH
芳香化酶细胞色素P-450酶在人类卵巢的细胞分布进行了研究免疫细胞化学，使用芳香化酶特异性单克隆抗体。从立即尸检或手术后获得年龄范围从青春期前女婴到绝经后成年的女性卵巢。结果揭示了芳香化酶在不同发育阶段表达的时空变化。在2.5个月婴儿的卵巢中未检测到免疫反应性芳香化酶。在绝经前的卵巢，芳香化酶是缺席的基质室，但在卵泡中，芳香化酶的表达一致的模式进行了观察，其大小和发育阶段。芳香化酶在原始卵泡、初级卵泡和直径小于250 μ m的次级卵泡中不表达。在稍大的卵泡（直径250-700 μ m）中，首先在一些卵泡膜细胞（TC）中检测到芳香酶。在更发达的次级卵泡到大的排卵前卵泡（> 1 cm）中，TC芳香化酶免疫染色的强度和阳性细胞的数量增加，并且反应定位于TI/外膜界面处的一条内膜（TI）细胞带。在颗粒细胞（GC）中，芳香化酶首先在窦形成的初始阶段（> 700 μ m）的卵泡中被检测到，并且随着卵泡直径和窦腔的增加，染色增强，在排卵前卵泡中最大。GC芳香化酶总是在TI免疫染色的存在下被发现。这两个细胞群被未染色的TI细胞层分开，使卵泡壁呈现带状外观。免疫染色在壁GC中最强，在胃窦GC细胞中较弱，在卵丘GC中不存在。免疫反应性芳香化酶也检测到功能性黄体（CL），但不存在从退化CL的和白色小体。我们的研究结果表明，免疫染色细胞的CL是由前GC和可能的前TI细胞的亚群。在围月经期卵巢中，没有任何滤泡或间质芳香化酶免疫染色的证据。在绝经后卵巢中没有观察到卵泡，但发现3/7个标本的基质室中的单个细胞和细胞簇具有芳香化酶免疫染色反应。在所有情况下，芳香酶免疫染色反应均为细胞质。该结果提供了TC芳香化酶和绝经后妇女基质细胞芳香化酶存在的第一个直接证据。
The cellular distribution of the aromatase cytochrome P-450 enzyme in human ovaries has been investigated immunocytochemically, using an aromatase-specific monoclonal antibody. Ovaries of females ranging in age from prepubertal infant girl through to postmenopausal adulthood were obtained from immediate autopsy or after surgery. The results have revealed temporal and spatial changes in expression of aromatase at different stages of development. No immunoreactive aromatase was detected in the ovary of the 2.5 month infant. In premenopausal ovaries, aromatase was absent from the stromal compartment, but in follicles, a consistent pattern in expression of aromatase was observed, related to their size and developmental stage. Aromatase was not expressed in primordial, primary, or small secondary follicles less than 250-mu-m diameter. In slightly larger follicles (250-700-mu-m diameter) aromatase was first detected in a few thecal cells (TC). In more developed secondary through to large preovulatory follicles (> 1 cm) TC aromatase immunostain increased in intensity and number of positive cells, and the reaction was localized to a band of theca interna (TI) cells at the TI/theca externa interface. In granulosa cells (GC), aromatase was first detected in follicles in the initial stages of antrum formation (> 700-mu-m), and staining intensified as follicle diameter and antral cavity increased, being maximal in preovulatory follicles. GC aromatase was always found in the presence of TI immunostain. These two cell populations were separated by an unstained layer of TI cells giving the follicle walls a banded appearance. Immunostain was most intense in mural GC, was weaker in antral GC cells and was absent from the cumulus GC. Immunoreactive aromatase was also detected in functional corpora lutea (CL) but was absent from involuting CL's and corpora albicans. Our findings indicate that the immunostained cells of the CL are comprised of the former GC and possibly a subpopulation of former TI cells. In perimenopausal ovaries there was no evidence of any follicular or stromal aromatase immunostain. In postmenopausal ovaries no follicles were observed, but individual cells and clusters of cells in the stromal compartment of 3/7 specimens were found to have an aromatase immunostain reaction. In all cases, the aromatase immunostain reaction was cytoplasmic. The results provide the first direct evidence of the existence of TC aromatase, and of stromal cell aromatase in postmenopausal women.