Synthesis and application of a macroporous boronate affinity monolithic column using a metal-organic gel as a porogenic template for the specific capture of glycoproteins.
Synthesis and application of a macroporous boronate affinity monolithic column using a metal-organic gel as a porogenic template for the specific capture of glycoproteins.
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DOI：
10.1016/j.chroma.2011.10.049
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发表时间：
2011-12
期刊：
影响因子：
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通讯作者：
Fan Yang;Zian Lin;Xiwen He;Langxing Chen;Yukui Zhang
中科院分区：
文献类型：
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作者：
Fan Yang;Zian Lin;Xiwen He;Langxing Chen;Yukui Zhang
制备了大孔硼酸盐亲和整体柱，并以金属有机凝胶（MOG）为致孔模板，用于特异性捕获糖蛋白。与传统的成孔方法相比，MOG的这种新探索的应用已被证明是形成大孔的更方便的方法。采用原位聚合法在不锈钢柱上合成了聚（3-丙烯酰胺基苯硼酸-co-乙烯基二甲基丙烯酸酯）整体柱。为了制备具有均匀开放通道网络的大孔结构，系统地研究了反应温度、MOG浓度和单体配比等制备条件。采用扫描电镜（SEM）和压汞法对所制备的大孔整体材料进行了表征。以辣根过氧化物酶（HRP）和转铁蛋白（TF）为受试糖蛋白，通过色谱分析表明，大孔硼酸盐亲和整体柱对糖蛋白的选择性和动态结合能力均优于非糖蛋白。所得到的亲和整体柱被成功地用于特异性捕获从牛血清样品中的TF。
A macroporous boronate affinity monolithic column was prepared and applied to specifically capture glycoproteins using metal-organic gels (MOGs) as a porogenic template. This newly explored application of MOGs has proven to be a more convenient method for the formation of macropores in contrast to traditional porogenic methods. The poly (3-acrylamidophenylboronic acid-co-ethylene dimethacrylate) monolithic columns were synthesized in stainless columns by in situ polymerization. To fabricate the macroporous formation with a uniformed open-channel network, the preparation conditions, such as reaction temperature, the concentration of the MOGs and the ratio of monomers were systematically investigated. The prepared macroporous monoliths were characterized by scanning electron microscope (SEM) and mercury intrusion porosimetry. Furthermore, horseradish peroxidase (HRP) and transferrin (TF) were chosen as test glycoproteins, and the chromatographic analysis demonstrated that the macroporous boronate affinity monoliths exhibited a higher selectivity and better dynamic binding capacity toward glycoproteins compared with non-glycoproteins. The resulted affinity monolithic column was successfully employed to specifically capture TF from a bovine serum sample.