A nonintrinsic regional basis for increased infrarenal aortic MMP-9 expression and activity
A nonintrinsic regional basis for increased infrarenal aortic MMP-9 expression and activity
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DOI：
10.1067/mva.2003.163
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发表时间：
2003-05-01
影响因子：
4.3
通讯作者：
Upchurch, GR
中科院分区：
文献类型：
--
作者：
Ailawadi, G;Knipp, BS;Upchurch, GR
目的：本研究旨在探讨主动脉不同解剖部位的内在因素或区域因素是否影响基质金属蛋白酶（MMPs）和金属蛋白酶组织抑制剂（TIMPs）的表达和活性。将Sprague-Dawley大鼠（n=22）的主动脉分为主动脉弓、胸降段和肾下腹段。用白细胞介素-1 β（IL-1 β）（2 ng/mL）刺激标本72小时。在单独的实验中，从供体大鼠的胸或腹主动脉将同系主动脉段移植到受体大鼠的肾下主动脉位置（每种n=12）。在4周时，从接受移植的大鼠中收获腹膜，切成弓形、胸部和移植的胸部或移植的腹部节段，并用IL-1 β刺激。进行逆转录聚合酶链反应、酶谱和反向酶谱以评估所有主动脉节段中的MMMP-9、MMP-2和TIMP-1。采用方差分析（ANOVA）和事后Tukey检验评估差异。对照组大鼠腹段MMP-9表达显著高于弓段和胸段（P
Objective: This investigation was undertaken to determine whether intrinsic or regional factors at different anatomic sites of the aorta affect expression and activity of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs).Methods. Aortas from Sprague-Dawley rats (n=22) were divided into arch, descending thoracic, and infrarenal abdominal segments. Specimens were stimulated with interleukin-1beta (IL-1beta) (2 ng/mL) for 72 hours. In separate experiments, syngeneic aortic segments were transplanted from the thoracic or abdominal aortas of donor rats into the infrarenal aortic position of recipient rats (n=12 each). At 4 weeks, aortas from rats who had received transplants were harvested, sectioned into arch, thoracic, and transplanted thoracic or transplanted abdominal segments, and stimulated with IL-1beta. Reverse transcriptase polymerase chain reaction, zymography, and reverse zymography were performed to assess MMMP-9, MMP-2, and TIMP-1 in all aortic segments. Differences were assessed with analysis of variance (ANOVA) and post-hoc Tukey test.Results. In control rats, abdominal segments had significantly higher MMP-9 expression compared with arch and thoracic segments (P