Aptameric sensors based on structural change for diagnosis.
Aptameric sensors based on structural change for diagnosis.
复制标题
基于结构变化的适体传感器进行诊断。
DOI：
10.1039/c005359g
复制
发表时间：
2011
影响因子：
3.4
通讯作者：
K. Ikebukuro
中科院分区：
文献类型：
--
作者：
K. Abe;Daisuke Ogasawara;W. Yoshida;K. Sode;K. Ikebukuro
适配体是一种可以与各种分子结合的核酸。因为适配体具有抗体所缺乏的一些特征，它们可以作为抗体的替代品。为了生物传感的目的，我们重点研究了与靶分子结合时发生结构变化的适体。我们构建了一个基于适配体的结合/游离（B/F）分离系统，该系统使用了一个设计好的适配体，命名为“可捕获适配体”。可捕获的适体在识别其靶分子后改变其结构，从而暴露特定的单链区域。与这个暴露区域互补的寡核苷酸，被称为“捕获DNA”，固定在一个支架上。这种设计允许捕获DNA捕获与靶标结合的适体，随后通过B/F分离去除任何未结合的适体和污染物。去除未结合的污染物或适配体的结果是高度敏感的检测，其水平与基于三明治的免疫分析法的检测水平相似。我们通过使用可捕获的适体描述了凝血酶检测系统的构建，并讨论了可捕获的适体在临床诊断中的潜力。
Aptamers are nucleic acids that can bind to various molecules. Because they have some features that are lacking in antibodies, aptamers could serve as alternatives to antibodies. For the purpose of biosensing, we focused on aptamers that undergo structural changes on binding to their target molecules. We constructed an aptamer-based bound/free (B/F) separation system that uses a designed aptamer named the "capturable aptamer". The capturable aptamer changes its structure upon recognizing its target molecule thereby exposing a specific single-strand region. The oligonucleotide that is complementary to this exposed region, named the "capture DNA" is immobilized on a support. This design permits the exclusive capture by the capture DNA of the aptamer bound to its target, and subsequent removal of any unbound aptamer and contaminants by B/F separation. The removal of unbound contaminants or aptamers results in highly sensitive detection at similar levels to those achievable by sandwich-based immunoassay. We describe the construction of a thrombin-detection system by using a capturable aptamer, and we discuss the potential of capturable aptamers in clinical diagnostics.
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